New Investigators must meet with the Core team to discuss their project before sample submission can be arranged. Investigators are expected to load samples onto plates following the instructions below. 

*Investigators can submit individual samples in tubes and request sample plate loading by the Core for an additional fee. 

A. Preparation for Olink run

  1. Email Core staff (AffinityProteomics@bch.harvard.edu) to discuss and arrange your study. 
  2. Prepare your sample manifest using the provided template. Please modify the manifest file name to include date, institute, user last name, and specified panel (i.e. T48_01012027_BCH_Lee_humancytokine_manifest). Fill out the highlighted cells with your sample ID. Data output will be aligned to the sample ID provided in the manifest.
  3. Confirm plan with Core to transfer Investigator-plated thawed samples on the scheduled date of sample submission, or transfer Investigator-plated frozen samples prior to scheduled date.
  4. Arrange date and time for sample transfer with Core staff (Monday to Thursday, 9 AM to 1 PM). 

B. Sample plating by Investigators

  1. Print out your sample manifest. Use this as a reference while you plate the samples.
  2. Prepare a biosafety cabinet, sterile pipette tips, and a polypropylene 96-well PCR plate. Please note: we will only accept Investigator-plated samples delivered in polypropylene PCR plates.  We will not accept samples delivered in flat-bottom, round-bottom, or V-bottom tissue culture plates. The Core can provide a PCR plate for sample plating upon request. 
  3. Pipette at least 10 µL of each sample into the PCR plate according to the following map. Observe sterile technique during plating to avoid contamination. After pipetting, seal the plate tightly with an aluminum adhesive film (ensure the film is compatible for cold storage to -80C). 

For Target-48 and Olink Flex panels, use the following arrangement for plate loading. Columns 6 to 12 should remain empty. 

 123456789101112
ASample 1Sample 2Sample 3Sample 4Sample 5       
BSample 6Sample 7Sample 8Sample 9Sample 10       
CSample 11Sample 12Sample 13Sample 14Sample 15       
DSample 16Sample 17Sample 18Sample 19Sample 20       
ESample 21Sample 22Sample 23Sample 24Sample 25       
FSample 26Sample 27Sample 28Sample 29Sample 30       
GSample 31Sample 32Sample 33Sample 34Sample 35       
HSample 36Sample 37Sample 38Sample 39Sample 40       
             

For Target-96 panels, use the following arrangement for plate loading. Column 12 should remain empty. 

 123456789101112
ASample 1Sample 2Sample 3Sample 4Sample 5Sample 6Sample 7Sample 8Sample 9Sample 10Sample 11 
BSample 12Sample 13Sample 14Sample 15Sample 16Sample 17Sample 18Sample 19Sample 20Sample 21Sample 22 
CSample 23Sample 24Sample 25Sample 26Sample 27Sample 28Sample 29Sample 30Sample 31Sample 32Sample 33 
DSample 34Sample 35Sample 36Sample 37Sample 38Sample 39Sample 40Sample 41Sample 42Sample 43Sample 44 
ESample 45Sample 46Sample 47Sample 48Sample 49Sample 50Sample 51Sample 52Sample 53Sample 54Sample 55 
FSample 56Sample 57Sample 58Sample 59Sample 60Sample 61Sample 62Sample 63Sample 64Sample 65Sample 66 
GSample 67Sample 68Sample 69Sample 70Sample 71Sample 72Sample 73Sample 74Sample 75Sample 76Sample 77 
HSample 78Sample 79Sample 80Sample 81Sample 82Sample 83Sample 84Sample 85Sample 86Sample 87Sample 88 

C. Sample deliver and pick up

  1. Investigators will deliver the plated samples to the Core on ice or dry ice based on the specified study arrangements. 
    • Core Address: 1 Blackfan circle, Karp Research Building RB-10006A, Boston, MA 02464
    • Telephone: 617-919-5008
  2. Remaining samples can be picked up by Investigators within 30 days after study completion.