Boston Children’s TEG Coagulation Laboratory focuses on improving the prediction and management of perioperative bleeding and thrombosis in pediatric patients undergoing complex cardiac surgery and extracorporeal membrane oxygenation (ECMO). Our research evaluates advanced coagulation monitoring tools to support evidence-based, goal-directed hemostatic management across these high-risk patient populations. 

Areas of focus include:

  • Assessment of existing FDA approved point-of-care diagnostic testing devices to improve customized anticoagulation management for cardiac patients
  • Development of novel point-of-care coagulation diagnostic tools currently unavailable in clinical practice
  • Identification of key areas for clinical investigation, including prospective and retrospective coagulation studies

As part of this work, we validated multiple thromboelastography (TEG) platforms, including the mechanical TEG5000 and the point-of-care optical TEG6S platforms. Our findings demonstrate that viscoelastic-guided transfusion strategies are associated with improved bleeding control and reduced exposure to unnecessary blood products. Additionally, viscoelastic parameters indicative of reduced clot strength during surgery were associated with increased bleeding risk, while distinct coagulation profiles observed in patients receiving extracorporeal support suggested a propensity toward hypercoagulability.

In parallel, we investigated platelet function testing using VerifyNow to assess aspirin responsiveness in pediatric cardiac surgery patients. Aspirin unresponsiveness was significantly associated with increased postoperative thrombosis in select cardiac populations, whereas aspirin dose escalation in nonresponsive patients correlated with a reduced risk of thrombotic events. Together, these findings support the use of VerifyNow-guided aspirin monitoring to optimize thromboprophylaxis and reduce postoperative thrombotic complications.

By combining clinical expertise with innovative research, the TEG Coagulation Laboratory is shaping the future of precision coagulation management in cardiac patient care.

Peer-reviewed publications

Research support

Ongoing research support

  • “Coagulation testing in pediatric patients Undergoing Cardiac Surgery” Departmental Funding  
    • Principal Investigator: Sirisha Emani
  • “Validation of newer version of a current diagnostic test to comply with BCH regulatory requirements” Haemonetics Inc.
    • Principal Investigator: Sirisha Emani

Completed research support

  • “Digital Microfluidics – Minimizing Blood Volume for Pediatric Coagulation Screening” NIH/NHLBI_ R44HL125484 Phase IIB
    • Principal Investigators: Vamsee Pamula PhD, and Sitaram M. Emani MD
  • “Improving Anticoagulation Monitoring in Pediatric Patients: Use of a Microfluidic Platform to Test Low Volume Blood Samples Obtained by Heel-Stick Collection.” 1 R44 HL 140662 Heparin Monitoring Fast-Track award
    • Principal Investigator: Sitaram M. Emani MD
  • “Near Patient Assessment of Thromboelastography and ROTEM in Pediatric Patients Undergoing Cardiac Surgery” Goldwin Foundation G3123445
    • Principal Investigator: Sitaram M. Emani MD
  • “Feasibility for utilizing T2MR for coagulation diagnostics in infants undergoing complex congenital cardiac surgery” T2 Biosystems Corp., Lexington, MA
    • Principal Investigator: Sitaram M. Emani MD
  • “Coagulation Panel Testing in Pediatric Patients Following Initiation of Extracorporeal Membrane Oxygenation (ECMO)” Olivia Heart Foundation Grant HL 150684
    • Principal Investigator: Sitaram M. Emani MD
  • “Digital Microfluidic Platform for Coagulation Diagnostic Testing” NIH SBIR / HHSN268201000001C
    • Principal Investigators: Vamsee Pamula PhD, and Sitaram M. Emani MD
  • “Multiplex hypercoagulability panel on a digital microfluidic system” NIH subcontract HHSN268201000001C NHLBI 
    • Principal Investigators: Vamsee Pamula PhD, and Sitaram M. Emani MD