Information

Related Research Units

Megakaryocytes to Platelets Research Group Research
Vascular Biology Program Research

Research Overview

Welcome to the Italiano Lab. Our research uses cell and molecular biology methods to address problems in megakaryocyte and platelet biology. The lab’s research focuses primarily on how blood platelets, which function as the band-aids of the bloodstream, are produced from megakaryocyte precursor cells. Specifically, the lab uses mouse megakaryocyte and human culture systems to study platelet production in vitro. Our primary methods include fluorescence microscopy, live cell imaging, molecular biology, biochemistry, electron microscopy, bioengineering, and knockout mice. Where possible, we attempt to study the dynamics of proteins in living megakaryocytes or reconstitute cellular process with cell extracts. Our lab has demonstrated that platelet formation follows a defined set of morphogenetic shape changes driven by forces derived from both microtubules and actin filaments. Current focuses include understanding the molecular signals that trigger platelet production, using biologically inspired engineering to establish how the bone marrow microenvironment influences platelet production, understanding how the cytoskeleton powers platelet production. We also have a major interest in understanding the non-hemostatic roles of platelets in health and disease. This includes establishing how platelets regulate new blood vessel growth, immunity, cancer, wound healing, and potentially aging..

 

Research Background

Joseph Italiano is an Associate Professor who holds academic appointments at Boston Children’s Hospital and Harvard Medical School. He was awarded his Ph.D. in Cell Biology from Florida State University, under the supervision of Thomas Roberts. His thesis focused on how filament assembly and bundling contribute towards cell crawling in amoeboid sperm of Ascaris. Since then, Dr. Italiano transitioned into hematology-based research with John Hartwig at Brigham and Women’s Hospital, where he first began studying the cytoskeletal mechanisms of blood platelet formation, which remains a focus of his work as a Principal Investigator at the Boston Children's Hospital

 

Publications

  1. Serglycin controls megakaryocyte retention of platelet factor 4 and influences megakaryocyte fate in bone marrow. Blood Adv. 2025 Jan 14; 9(1):15-28. View Abstract
  2. Looking Under the Hood at the Cytoskeletal Engine of Platelet Production. Arterioscler Thromb Vasc Biol. 2025 Feb; 45(2):186-197. View Abstract
  3. Inhibition of RhoA-mediated secretory autophagy in megakaryocytes mitigates myelofibrosis in mice. bioRxiv. 2024 Dec 05. View Abstract
  4. Shear Stress Promotes Remodeling of Platelet Glycosylation via Upregulation of Platelet Glycosidase Activity: One More Thing. Thromb Haemost. 2024 Sep 12. View Abstract
  5. CpG oligonucleotides induce acute murine thrombocytopenia dependent on toll-like receptor 9 and spleen tyrosine kinase pathways. J Thromb Haemost. 2024 Nov; 22(11):3266-3276. View Abstract
  6. Targeting cargo to an unconventional secretory system within megakaryocytes allows the release of transgenic proteins from platelets. J Thromb Haemost. 2024 Nov; 22(11):3235-3248. View Abstract
  7. What It Takes To Be a Platelet: Evolving Concepts in Platelet Production. Circ Res. 2024 Aug 02; 135(4):540-549. View Abstract
  8. Evidence for a cytoplasmic proplatelet promoting factor that triggers platelet production. Haematologica. 2024 07 01; 109(7):2341-2345. View Abstract
  9. Cell cycle-dependent centrosome clustering precedes proplatelet formation. Sci Adv. 2024 Jun 21; 10(25):eadl6153. View Abstract
  10. Dynamic actin/septin network in megakaryocytes coordinates proplatelet elaboration. Haematologica. 2024 03 01; 109(3):915-928. View Abstract
  11. The bone marrow is the primary site of thrombopoiesis. Blood. 2024 01 18; 143(3):272-278. View Abstract
  12. Thrombopoietin levels in sepsis and septic shock - a systematic review and meta-analysis. Clin Chem Lab Med. 2024 Apr 25; 62(5):999-1010. View Abstract
  13. Efficient megakaryopoiesis and platelet production require phospholipid remodeling and PUFA uptake through CD36. Nat Cardiovasc Res. 2023 Aug; 2(8):746-763. View Abstract
  14. Sodium bicarbonate as a local adjunctive agent for limiting platelet activation, aggregation, and adhesion within cardiovascular therapeutic devices. J Thromb Thrombolysis. 2023 Oct; 56(3):398-410. View Abstract
  15. A Critical Role for ERO1a in Arterial Thrombosis and Ischemic Stroke. Circ Res. 2023 05 26; 132(11):e206-e222. View Abstract
  16. Spatial transcriptomics of murine bone marrow megakaryocytes at single-cell resolution. Res Pract Thromb Haemost. 2023 May; 7(4):100158. View Abstract
  17. Shear-Mediated Platelet Microparticles Demonstrate Phenotypic Heterogeneity as to Morphology, Receptor Distribution, and Hemostatic Function. Int J Mol Sci. 2023 Apr 17; 24(8). View Abstract
  18. Proceedings of the immune thrombocytopenia summit: new concepts in mechanisms, diagnosis, and management. Res Pract Thromb Haemost. 2023 Feb; 7(2):100097. View Abstract
  19. Efficient megakaryopoiesis and platelet production require phospholipid remodeling and PUFA uptake through CD36. bioRxiv. 2023 Feb 12. View Abstract
  20. Shear-Mediated Platelet Microparticles Demonstrate Phenotypic Heterogeneity as to Morphology, Receptor Distribution, and Hemostatic Function. bioRxiv. 2023 Feb 08. View Abstract
  21. Platelets upregulate tumor cell programmed death ligand 1 in an epidermal growth factor receptor-dependent manner in vitro. Blood Adv. 2022 10 25; 6(20):5668-5675. View Abstract
  22. Pro-inflammatory megakaryocyte gene expression in murine models of breast cancer. Sci Adv. 2022 10 14; 8(41):eabo5224. View Abstract
  23. DNA Origami-Platelet Adducts: Nanoconstruct Binding without Platelet Activation. Bioconjug Chem. 2022 07 20; 33(7):1295-1310. View Abstract
  24. Don't you forget about me(gakaryocytes). Blood. 2022 06 02; 139(22):3245-3254. View Abstract
  25. The triple crown of platelet generation. Blood. 2022 04 07; 139(14):2100-2101. View Abstract
  26. Sequence-specific 2'-O-methoxyethyl antisense oligonucleotides activate human platelets through glycoprotein VI, triggering formation of platelet-leukocyte aggregates. Haematologica. 2022 02 01; 107(2):519-531. View Abstract
  27. The secreted tyrosine kinase VLK is essential for normal platelet activation and thrombus formation. Blood. 2022 01 06; 139(1):104-117. View Abstract
  28. Microvesicles, but not platelets, bud off from mouse bone marrow megakaryocytes. Blood. 2021 11 18; 138(20):1998-2001. View Abstract
  29. Evolving perspectives on mechanical circulatory support biocompatibility and interfaces. Ann Cardiothorac Surg. 2021 May; 10(3):396-398. View Abstract
  30. Shear-mediated platelet activation in the free flow II: Evolving mechanobiological mechanisms reveal an identifiable signature of activation and a bi-directional platelet dyscrasia with thrombotic and bleeding features. J Biomech. 2021 06 23; 123:110415. View Abstract
  31. Transfer to the clinic: refining forward programming of hPSCs to megakaryocytes for platelet production in bioreactors. Blood Adv. 2021 04 13; 5(7):1977-1990. View Abstract
  32. Platelet Dysfunction During Mechanical Circulatory Support: Elevated Shear Stress Promotes Downregulation of aIIbß3 and GPIb via Microparticle Shedding Decreasing Platelet Aggregability. Arterioscler Thromb Vasc Biol. 2021 04; 41(4):1319-1336. View Abstract
  33. VWF maturation and release are controlled by 2 regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2. Blood. 2020 12 10; 136(24):2824-2837. View Abstract
  34. High-content, label-free analysis of proplatelet production from megakaryocytes. J Thromb Haemost. 2020 10; 18(10):2701-2711. View Abstract
  35. Platelet Dysfunction and Thrombosis in JAK2V617F-Mutated Primary Myelofibrotic Mice. Arterioscler Thromb Vasc Biol. 2020 10; 40(10):e262-e272. View Abstract
  36. Platelet-derived extracellular vesicles infiltrate and modify the bone marrow during inflammation. Blood Adv. 2020 07 14; 4(13):3011-3023. View Abstract
  37. Sniffing out the aroma(tase) of drug-induced thrombocytopenia. Blood. 2020 06 11; 135(24):2116-2117. View Abstract
  38. Platelet Activation via Shear Stress Exposure Induces a Differing Pattern of Biomarkers of Activation versus Biochemical Agonists. Thromb Haemost. 2020 May; 120(5):776-792. View Abstract
  39. Yin and Yang of MCS-Related Coagulopathy: Shear Stress Promotes Platelet Prothrombosis and Microparticle Generation While Inducing Integrin Downregulation and Decreased Aggregability. J Heart Lung Transplant. 2020 Apr; 39(4S):S177. View Abstract
  40. Platelet alloantibody detection: moving ahead. Blood. 2019 11 28; 134(22):1887-1888. View Abstract
  41. Megakaryocyte Reprogramming in Breast Cancer. Blood. 2019 Nov 13; 134(Supplement_1):12. View Abstract
  42. VWF Exocytosis and Biogenesis of Weibel Palade Bodies in Endothelial Cells Are Differentially Controlled By Interactions between Bloc-2 and the Exocyst Complex. Blood. 2019 Nov 13; 134(Supplement_1):8. View Abstract
  43. Megakaryocytes package contents into separate a-granules that are differentially distributed in platelets. Blood Adv. 2019 10 22; 3(20):3092-3098. View Abstract
  44. Megakaryocyte emperipolesis mediates membrane transfer from intracytoplasmic neutrophils to platelets. Elife. 2019 05 01; 8. View Abstract
  45. Anti-apoptotic BCL2L2 increases megakaryocyte proplatelet formation in cultures of human cord blood. Haematologica. 2019 10; 104(10):2075-2083. View Abstract
  46. Aspirin inhibits platelets from reprogramming breast tumor cells and promoting metastasis. Blood Adv. 2019 01 22; 3(2):198-211. View Abstract
  47. Unlocking the Molecular Secrete(s) of a-Granule Biogenesis. Arterioscler Thromb Vasc Biol. 2018 11; 38(11):2539-2541. View Abstract
  48. Platelets release pathogenic serotonin and return to circulation after immune complex-mediated sequestration. Proc Natl Acad Sci U S A. 2018 02 13; 115(7):E1550-E1559. View Abstract
  49. Developmental Stage-Specific Manifestations of Absent TPO/c-MPL Signalling in Newborn Mice. Thromb Haemost. 2017 12; 117(12):2322-2333. View Abstract
  50. Mature murine megakaryocytes present antigen-MHC class I molecules to T cells and transfer them to platelets. Blood Adv. 2017 Sep 12; 1(20):1773-1785. View Abstract
  51. Deletion of the Arp2/3 complex in megakaryocytes leads to microthrombocytopenia in mice. Blood Adv. 2017 Aug 08; 1(18):1398-1408. View Abstract
  52. Selinexor-induced thrombocytopenia results from inhibition of thrombopoietin signaling in early megakaryopoiesis. Blood. 2017 08 31; 130(9):1132-1143. View Abstract
  53. Megakaryocytes compensate for Kit insufficiency in murine arthritis. J Clin Invest. 2017 May 01; 127(5):1714-1724. View Abstract
  54. Tamoxifen Directly Inhibits Platelet Angiogenic Potential and Platelet-Mediated Metastasis. Arterioscler Thromb Vasc Biol. 2017 04; 37(4):664-674. View Abstract
  55. DREAM plays an important role in platelet activation and thrombogenesis. Blood. 2017 01 12; 129(2):209-225. View Abstract
  56. Human thrombopoiesis depends on Protein kinase Cd/protein kinase Ce functional couple. Haematologica. 2016 07; 101(7):812-20. View Abstract
  57. Lysyl oxidase is associated with increased thrombosis and platelet reactivity. Blood. 2016 Mar 17; 127(11):1493-501. View Abstract
  58. Synthesis and dephosphorylation of MARCKS in the late stages of megakaryocyte maturation drive proplatelet formation. Blood. 2016 Mar 17; 127(11):1468-80. View Abstract
  59. CCL5 derived from platelets increases megakaryocyte proplatelet formation. Blood. 2016 Feb 18; 127(7):921-6. View Abstract
  60. Cytoskeletal perturbation leads to platelet dysfunction and thrombocytopenia in variant forms of Glanzmann thrombasthenia. Haematologica. 2016 Jan; 101(1):46-56. View Abstract
  61. Abnormal megakaryopoiesis and platelet function in cyclooxygenase-2-deficient mice. Thromb Haemost. 2015 Nov 25; 114(6):1218-29. View Abstract
  62. Road blocks in making platelets for transfusion. J Thromb Haemost. 2015 Jun; 13 Suppl 1:S55-62. View Abstract
  63. Inducible Gata1 suppression expands megakaryocyte-erythroid progenitors from embryonic stem cells. J Clin Invest. 2015 Jun; 125(6):2369-74. View Abstract
  64. IL-1a induces thrombopoiesis through megakaryocyte rupture in response to acute platelet needs. J Cell Biol. 2015 May 11; 209(3):453-66. View Abstract
  65. Microtubule sliding drives proplatelet elongation and is dependent on cytoplasmic dynein. Blood. 2015 Jan 29; 125(5):860-8. View Abstract
  66. Scalable generation of universal platelets from human induced pluripotent stem cells. Stem Cell Reports. 2014 Nov 11; 3(5):817-31. View Abstract
  67. Platelet bioreactor-on-a-chip. Blood. 2014 Sep 18; 124(12):1857-67. View Abstract
  68. A secreted tyrosine kinase acts in the extracellular environment. Cell. 2014 Aug 28; 158(5):1033-1044. View Abstract
  69. Proteasome function is required for platelet production. J Clin Invest. 2014 Sep; 124(9):3757-66. View Abstract
  70. Platelet bioreactor-on-a-chip. Blood. 2014 Jul 21. View Abstract
  71. Expansion of the neonatal platelet mass is achieved via an extension of platelet lifespan. Blood. 2014 May 29; 123(22):3381-9. View Abstract
  72. Interpreting the developmental dance of the megakaryocyte: a review of the cellular and molecular processes mediating platelet formation. Br J Haematol. 2014 Apr; 165(2):227-36. View Abstract
  73. Macrocytic anemia and mitochondriopathy resulting from a defect in sideroflexin 4. Am J Hum Genet. 2013 Nov 07; 93(5):906-14. View Abstract
  74. Anticoagulation inhibits tumor cell-mediated release of platelet angiogenic proteins and diminishes platelet angiogenic response. Blood. 2014 Jan 02; 123(1):101-12. View Abstract
  75. Animating platelet production adds physiological context. Trends Mol Med. 2013 Oct; 19(10):583-5. View Abstract
  76. Proplatelet generation in the mouse requires PKCe-dependent RhoA inhibition. Blood. 2013 Aug 15; 122(7):1305-11. View Abstract
  77. The incredible journey: From megakaryocyte development to platelet formation. J Cell Biol. 2013 Jun 10; 201(6):785-96. View Abstract
  78. Dynamin-related protein-1 controls fusion pore dynamics during platelet granule exocytosis. Arterioscler Thromb Vasc Biol. 2013 Mar; 33(3):481-8. View Abstract
  79. Unraveling mechanisms that control platelet production. Semin Thromb Hemost. 2013 Feb; 39(1):15-24. View Abstract
  80. Netrin-1 promotes glioblastoma cell invasiveness and angiogenesis by multiple pathways including activation of RhoA, cathepsin B, and cAMP-response element-binding protein. J Biol Chem. 2013 Jan 25; 288(4):2210-22. View Abstract
  81. Canonical Wnt signaling in megakaryocytes regulates proplatelet formation. Blood. 2013 Jan 03; 121(1):188-96. View Abstract
  82. The microtubule plus-end tracking protein CLASP2 is required for hematopoiesis and hematopoietic stem cell maintenance. Cell Rep. 2012 Oct 25; 2(4):781-8. View Abstract
  83. A GWAS sequence variant for platelet volume marks an alternative DNM3 promoter in megakaryocytes near a MEIS1 binding site. Blood. 2012 Dec 06; 120(24):4859-68. View Abstract
  84. Differential roles of cAMP and cGMP in megakaryocyte maturation and platelet biogenesis. Exp Hematol. 2013 Jan; 41(1):91-101.e4. View Abstract
  85. Altered microtubule equilibrium and impaired thrombus stability in mice lacking RanBP10. Blood. 2012 Oct 25; 120(17):3594-602. View Abstract
  86. T granules in human platelets function in TLR9 organization and signaling. J Cell Biol. 2012 Aug 20; 198(4):561-74. View Abstract
  87. MKL1 and MKL2 play redundant and crucial roles in megakaryocyte maturation and platelet formation. Blood. 2012 Sep 13; 120(11):2317-29. View Abstract
  88. High-content live-cell imaging assay used to establish mechanism of trastuzumab emtansine (T-DM1)--mediated inhibition of platelet production. Blood. 2012 Sep 06; 120(10):1975-84. View Abstract
  89. Does size matter in platelet production? Blood. 2012 Aug 23; 120(8):1552-61. View Abstract
  90. Microtubule and cortical forces determine platelet size during vascular platelet production. Nat Commun. 2012 May 22; 3:852. View Abstract
  91. The origin and function of platelet glycosyltransferases. Blood. 2012 Jul 19; 120(3):626-35. View Abstract
  92. VEGF, PF4 and PDGF are elevated in platelets of colorectal cancer patients. Angiogenesis. 2012 Jun; 15(2):265-73. View Abstract
  93. Visualization and manipulation of the platelet and megakaryocyte cytoskeleton. Methods Mol Biol. 2012; 788:109-25. View Abstract
  94. Platelets: production, morphology and ultrastructure. Handb Exp Pharmacol. 2012; (210):3-22. View Abstract
  95. Release of angiogenesis regulatory proteins from platelet alpha granules: modulation of physiologic and pathologic angiogenesis. Blood. 2011 Aug 04; 118(5):1359-69. View Abstract
  96. The identification and characterization of zebrafish hematopoietic stem cells. Blood. 2011 Jul 14; 118(2):289-97. View Abstract
  97. The spectrin-based membrane skeleton stabilizes mouse megakaryocyte membrane systems and is essential for proplatelet and platelet formation. Blood. 2011 Aug 11; 118(6):1641-52. View Abstract
  98. Platelets and the immune continuum. Nat Rev Immunol. 2011 Apr; 11(4):264-74. View Abstract
  99. Developmental differences in megakaryocytopoiesis are associated with up-regulated TPO signaling through mTOR and elevated GATA-1 levels in neonatal megakaryocytes. Blood. 2011 Apr 14; 117(15):4106-17. View Abstract
  100. Platelets generated from human embryonic stem cells are functional in vitro and in the microcirculation of living mice. Cell Res. 2011 Mar; 21(3):530-45. View Abstract
  101. Cytoskeletal mechanics of proplatelet maturation and platelet release. J Cell Biol. 2010 Nov 15; 191(4):861-74. View Abstract
  102. Platelet- and megakaryocyte-derived microparticles. Semin Thromb Hemost. 2010 Nov; 36(8):881-7. View Abstract
  103. Clinical relevance of microparticles from platelets and megakaryocytes. Curr Opin Hematol. 2010 Nov; 17(6):578-84. View Abstract
  104. Normal ranges of angiogenesis regulatory proteins in human platelets. Am J Hematol. 2010 Jul; 85(7):487-93. View Abstract
  105. Platelet formation. Semin Hematol. 2010 Jul; 47(3):220-6. View Abstract
  106. Serum response factor is an essential transcription factor in megakaryocytic maturation. Blood. 2010 Sep 16; 116(11):1942-50. View Abstract
  107. Junín virus infection of human hematopoietic progenitors impairs in vitro proplatelet formation and platelet release via a bystander effect involving type I IFN signaling. PLoS Pathog. 2010 Apr 15; 6(4):e1000847. View Abstract
  108. Platelet-derived thrombospondin-1 is a critical negative regulator and potential biomarker of angiogenesis. Blood. 2010 Jun 03; 115(22):4605-13. View Abstract
  109. The mouse mutation "thrombocytopenia and cardiomyopathy" (trac) disrupts Abcg5: a spontaneous single gene model for human hereditary phytosterolemia/sitosterolemia. Blood. 2010 Feb 11; 115(6):1267-76. View Abstract
  110. Selective sorting of alpha-granule proteins. J Thromb Haemost. 2009 Jul; 7 Suppl 1:173-6. View Abstract
  111. Dynamin 3 participates in the growth and development of megakaryocytes. Exp Hematol. 2008 Dec; 36(12):1714-27. View Abstract
  112. Platelets actively sequester angiogenesis regulators. Blood. 2009 Mar 19; 113(12):2835-42. View Abstract
  113. Megakaryocyte-derived microparticles: direct visualization and distinction from platelet-derived microparticles. Blood. 2009 Jan 29; 113(5):1112-21. View Abstract
  114. ABL2/ARG tyrosine kinase mediates SEMA3F-induced RhoA inactivation and cytoskeleton collapse in human glioma cells. J Biol Chem. 2008 Oct 03; 283(40):27230-8. View Abstract
  115. Direct visualization of the endomitotic cell cycle in living megakaryocytes: differential patterns in low and high ploidy cells. Cell Cycle. 2008 Aug; 7(15):2352-6. View Abstract
  116. RanBP10 is a cytoplasmic guanine nucleotide exchange factor that modulates noncentrosomal microtubules. J Biol Chem. 2008 May 16; 283(20):14109-19. View Abstract
  117. Visualization of microtubule growth in living platelets reveals a dynamic marginal band with multiple microtubules. Blood. 2008 May 01; 111(9):4605-16. View Abstract
  118. Role of Epac1, an exchange factor for Rap GTPases, in endothelial microtubule dynamics and barrier function. Mol Biol Cell. 2008 Mar; 19(3):1261-70. View Abstract
  119. Angiogenesis is regulated by a novel mechanism: pro- and antiangiogenic proteins are organized into separate platelet alpha granules and differentially released. Blood. 2008 Feb 01; 111(3):1227-33. View Abstract
  120. Platelet-associated PF-4 as a biomarker of early tumor growth. Blood. 2008 Feb 01; 111(3):1201-7. View Abstract
  121. Dynamic visualization of thrombopoiesis within bone marrow. Science. 2007 Sep 21; 317(5845):1767-70. View Abstract
  122. Delivering new insight into the biology of megakaryopoiesis and thrombopoiesis. Curr Opin Hematol. 2007 Sep; 14(5):419-26. View Abstract
  123. Mechanics of proplatelet elaboration. J Thromb Haemost. 2007 Jul; 5 Suppl 1:18-23. View Abstract
  124. Cytoskeletal mechanisms for platelet production. Blood Cells Mol Dis. 2006 Mar-Apr; 36(2):99-103. View Abstract
  125. The biogenesis of platelets from megakaryocyte proplatelets. J Clin Invest. 2005 Dec; 115(12):3348-54. View Abstract
  126. Mechanisms of organelle transport and capture along proplatelets during platelet production. Blood. 2005 Dec 15; 106(13):4066-75. View Abstract
  127. Differential roles of microtubule assembly and sliding in proplatelet formation by megakaryocytes. Blood. 2005 Dec 15; 106(13):4076-85. View Abstract
  128. Elevated release of sCD40L from platelets of diabetic patients by thrombin, glucose and advanced glycation end products. Diab Vasc Dis Res. 2005 May; 2(2):81-7. View Abstract
  129. Interactions between the megakaryocyte/platelet-specific beta1 tubulin and the secretory leukocyte protease inhibitor SLPI suggest a role for regulated proteolysis in platelet functions. Blood. 2004 Dec 15; 104(13):3949-57. View Abstract
  130. A role for Rab27b in NF-E2-dependent pathways of platelet formation. Blood. 2003 Dec 01; 102(12):3970-9. View Abstract
  131. The birth of the platelet. J Thromb Haemost. 2003 Jul; 1(7):1580-6. View Abstract
  132. Role for phosphoinositide 3-kinase in Fc gamma RIIA-induced platelet shape change. Am J Physiol Cell Physiol. 2003 Oct; 285(4):C797-805. View Abstract
  133. Megakaryocytes and beyond: the birth of platelets. J Thromb Haemost. 2003 Jun; 1(6):1174-82. View Abstract
  134. Alpha-adducin dissociates from F-actin and spectrin during platelet activation. J Cell Biol. 2003 May 12; 161(3):557-70. View Abstract
  135. Mechanisms and implications of platelet discoid shape. Blood. 2003 Jun 15; 101(12):4789-96. View Abstract
  136. Importance of free actin filament barbed ends for Arp2/3 complex function in platelets and fibroblasts. Proc Natl Acad Sci U S A. 2002 Dec 24; 99(26):16782-7. View Abstract
  137. A lineage-restricted and divergent beta-tubulin isoform is essential for the biogenesis, structure and function of blood platelets. Curr Biol. 2001 Apr 17; 11(8):579-86. View Abstract
  138. How the assembly dynamics of the nematode major sperm protein generate amoeboid cell motility. Int Rev Cytol. 2001; 202:1-34. View Abstract
  139. Hematopoietic-specific beta 1 tubulin participates in a pathway of platelet biogenesis dependent on the transcription factor NF-E2. Blood. 2000 Aug 15; 96(4):1366-73. View Abstract
  140. Blood platelets are assembled principally at the ends of proplatelet processes produced by differentiated megakaryocytes. J Cell Biol. 1999 Dec 13; 147(6):1299-312. View Abstract
  141. Localized depolymerization of the major sperm protein cytoskeleton correlates with the forward movement of the cell body in the amoeboid movement of nematode sperm. J Cell Biol. 1999 Sep 06; 146(5):1087-96. View Abstract
  142. The elegant platelet: signals controlling actin assembly. Thromb Haemost. 1999 Aug; 82(2):392-8. View Abstract
  143. Amoeboid motility without actin: insights into the molecular mechanism of locomotion using the major sperm protein (MSP) of nematodes. Biol Bull. 1998 Jun; 194(3):342-3; discussion 343-4. View Abstract
  144. Reconstitution in vitro of the motile apparatus from the amoeboid sperm of Ascaris shows that filament assembly and bundling move membranes. Cell. 1996 Jan 12; 84(1):105-14. View Abstract
  145. In Ascaris sperm pseudopods, MSP fibers move proximally at a constant rate regardless of the forward rate of cellular translocation. Cell Motil Cytoskeleton. 1995; 31(3):241-53. View Abstract

Contact Joseph E. Italiano, Jr.

Phone: 617-919-5124
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