Hanno Steen is a pioneer in developing methods for the qualitative and quantitative analysis of protein modifications by mass spectrometry, with special emphasis on tyrosine phosphorylation. In carcinogenesis, the pivotal role of tyrosine phosphorylation is underscored by the notion that almost half of the oncogenes known to date encode tyrosine kinases.
Hanno Steen's interests are focused on proteomics and biological mass spectrometry in general. He is particularly interested in the mass spectrometric analysis of the composition of complex protein mixtures, which includes not only the cataloging but also the comprehensive characterization of the components.
Dr. Steen's lab is working on novel methods for identifying and quantifying various protein modifications that are involved in pediatric diseases in order to provide new insights into the underlying biological processes, a prerequisite for a rational approach to prevent and/or cure these diseases.
Another goal of the lab's research is the development and applications of screening methods to detect and identify diagnostic and prognostic disease markers in body fluids, which offer an easily accessible mirror of the state of the entire body. These biomarkers can guide treatment decisions, predict patient phenotypes, and allow for early diagnosis.
Research Background
Dr. Steen studied chemistry at the University of Freiburg (Germany), UMIST/Manchester (UK), and Swiss Federal Institute of Technology (ETH) Zurich (Switzerland). He received his PhD in Biochemistry and Molecular Biology from the University of Southern Denmark, working in the laboratory of Dr. Matthias Mann on the mass spectrometric analysis of protein modifications. He completed postdoctoral fellowships in the laboratories of Drs. Steve Gygi and Marc Kirschner in the Departments of Cell Biology and Systems Biology at Harvard Medical School.
In 2004, Dr. Steen joined the faculty at Harvard Medical School and became principal investigator in the Department of Pathology at Boston Children's Hospital. Dr. Steen has published more than 180 papers in international peer-reviewed journals, which have been cited more than 23,000 times, resulting in an h-index of 66.
Dementia with lewy bodies patients with high tau levels display unique proteome profiles. Mol Neurodegener. 2024 Dec 19; 19(1):98. View Abstract
Chronic Viral Reactivation and Associated Host Immune Response and Clinical Outcomes in Acute COVID-19 and Post-Acute Sequelae of COVID-19. bioRxiv. 2024 Nov 16. View Abstract
The BNT162b2 mRNA vaccine demonstrates reduced age-associated TH1 support in vitro and in vivo. iScience. 2024 Nov 15; 27(11):111055. View Abstract
Plasma adenosine deaminase-1 and -2 activities are lower at birth in Papua New Guinea than in The Gambia but converge over the first weeks of life. Front Immunol. 2024; 15:1425349. View Abstract
PEPerMINT: peptide abundance imputation in mass spectrometry-based proteomics using graph neural networks. Bioinformatics. 2024 09 01; 40(Suppl 2):ii70-ii78. View Abstract
Longitudinal plasma proteomic analysis of 1117 hospitalized patients with COVID-19 identifies features associated with severity and outcomes. Sci Adv. 2024 May 24; 10(21):eadl5762. View Abstract
Integrated longitudinal multiomics study identifies immune programs associated with acute COVID-19 severity and mortality. J Clin Invest. 2024 May 01; 134(9). View Abstract
Diagnostic and Prognostic Biomarkers of Chronic Pancreatitis: A Conceptual Framework Based on the PRoBE Design. Gastroenterology. 2024 06; 166(6):957-962.e3. View Abstract
Quantitative profiling of posttranslational modifications of pathological tau via sarkosyl fractionation and mass spectrometry. Nat Protoc. 2024 Apr; 19(4):1235-1251. View Abstract
Analytical challenges in omics research on asthma and allergy: A National Institute of Allergy and Infectious Diseases workshop. J Allergy Clin Immunol. 2024 Apr; 153(4):954-968. View Abstract
Features of acute COVID-19 associated with post-acute sequelae of SARS-CoV-2 phenotypes: results from the IMPACC study. Nat Commun. 2024 Jan 03; 15(1):216. View Abstract
Integrated longitudinal multi-omics study identifies immune programs associated with COVID-19 severity and mortality in 1152 hospitalized participants. bioRxiv. 2023 Nov 06. View Abstract
Early cellular and molecular signatures correlate with severity of West Nile virus infection. iScience. 2023 Dec 15; 26(12):108387. View Abstract
Proteomics-Based Mapping of Bronchopulmonary Dysplasia-Associated Changes in Noninvasively Accessible Oral Secretions. J Pediatr. 2024 Jul; 270:113774. View Abstract
Multi-omic longitudinal study reveals immune correlates of clinical course among hospitalized COVID-19 patients. Cell Rep Med. 2023 06 20; 4(6):101079. View Abstract
Plasma proteome perturbation for CMV DNAemia in kidney transplantation. PLoS One. 2023; 18(5):e0285870. View Abstract
Integrative systems biology characterizes immune-mediated neurodevelopmental changes in murine Zika virus microcephaly. iScience. 2023 Jul 21; 26(7):106909. View Abstract
Meta-analysis of published cerebrospinal fluid proteomics data identifies and validates metabolic enzyme panel as Alzheimer's disease biomarkers. Cell Rep Med. 2023 04 18; 4(4):101005. View Abstract
A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics. Sci Adv. 2023 03 29; 9(13):eadf9717. View Abstract
Common mouse models of tauopathy reflect early but not late human disease. Mol Neurodegener. 2023 02 02; 18(1):10. View Abstract
The mRNA vaccine BNT162b2 demonstrates impaired TH1 immunogenicity in human elders in vitro and aged mice in vivo. Res Sq. 2022 Dec 21. View Abstract
Using CSF Proteomics to Investigate Herpesvirus Infections of the Central Nervous System. Viruses. 2022 12 10; 14(12). View Abstract
Longitudinal serum proteomics analyses identify unique and overlapping host response pathways in Lyme disease and West Nile virus infection. Front Immunol. 2022; 13:1012824. View Abstract
A Parallelization Strategy for the Time Efficient Analysis of Thousands of LC/MS Runs in High-Performance Computing Environment. J Proteome Res. 2022 11 04; 21(11):2810-2814. View Abstract
CAF08 adjuvant enables single dose protection against respiratory syncytial virus infection in murine newborns. Nat Commun. 2022 08 02; 13(1):4234. View Abstract
Using plasma proteomics to investigate viral infections of the central nervous system including patients with HIV-associated neurocognitive disorders. J Neurovirol. 2022 06; 28(3):341-354. View Abstract
Bacille Calmette-Guérin vaccine reprograms human neonatal lipid metabolism in vivo and in vitro. Cell Rep. 2022 05 03; 39(5):110772. View Abstract
Urine Proteomics Reveals Sex-Specific Response to Total Pancreatectomy With Islet Autotransplantation. Pancreas. 2022 05 01; 51(5):435-444. View Abstract
Plasma Proteomic Analysis Distinguishes Severity Outcomes of Human Ebola Virus Disease. mBio. 2022 06 28; 13(3):e0056722. View Abstract
Determinants of B-Cell Compartment Hyperactivation in European Adolescents Living With Perinatally Acquired HIV-1 After Over 10 Years of Suppressive Therapy. Front Immunol. 2022; 13:860418. View Abstract
multiFLEX-LF: A Computational Approach to Quantify the Modification Stoichiometries in Label-Free Proteomics Data Sets. J Proteome Res. 2022 04 01; 21(4):899-909. View Abstract
Urine Proteomics for Noninvasive Monitoring of Biomarkers in Bronchopulmonary Dysplasia. Neonatology. 2022; 119(2):193-203. View Abstract
Proteomics based markers of clinical pain severity in juvenile idiopathic arthritis. Pediatr Rheumatol Online J. 2022 Jan 15; 20(1):3. View Abstract
Using 'Big Data' to Disentangle Aging and COVID-19. Nat Aging. 2021 06; 1(6):496-497. View Abstract
Biomarkers of Chronic Pancreatitis: A systematic literature review. Pancreatology. 2021 Mar; 21(2):323-333. View Abstract
Sample Preparation for High-Throughput Urine Proteomics Using 96-Well Polyvinylidene Fluoride (PVDF) Membranes. Adv Exp Med Biol. 2021; 1306:1-12. View Abstract
Delayed Processing of Secretin-Induced Pancreas Fluid Influences the Quality and Integrity of Proteins and Nucleic Acids. Pancreas. 2021 01 01; 50(1):17-28. View Abstract
FLEXIQuant-LF to quantify protein modification extent in label-free proteomics data. Elife. 2020 12 07; 9. View Abstract
Corrigendum: Clinical Protocol for a Longitudinal Cohort Study Employing Systems Biology to Identify Markers of Vaccine Immunogenicity in Newborn Infants in The Gambia and Papua New Guinea. Front Pediatr. 2020; 8:610461. View Abstract
Tau PTM Profiles Identify Patient Heterogeneity and Stages of Alzheimer's Disease. Cell. 2020 12 10; 183(6):1699-1713.e13. View Abstract
Author Correction: PGBD5 promotes site-specific oncogenic mutations in human tumors. Nat Genet. 2020 Nov; 52(11):1265. View Abstract
Preparing for Life: Plasma Proteome Changes and Immune System Development During the First Week of Human Life. Front Immunol. 2020; 11:578505. View Abstract
Antioxidant functions of DHHC3 suppress anti-cancer drug activities. Cell Mol Life Sci. 2021 Mar; 78(5):2341-2353. View Abstract
Phosphorylation of ACTN4 Leads to Podocyte Vulnerability and Proteinuric Glomerulosclerosis. J Am Soc Nephrol. 2020 07; 31(7):1479-1495. View Abstract
Clinical Protocol for a Longitudinal Cohort Study Employing Systems Biology to Identify Markers of Vaccine Immunogenicity in Newborn Infants in The Gambia and Papua New Guinea. Front Pediatr. 2020; 8:197. View Abstract
Protein kinases PknA and PknB independently and coordinately regulate essential Mycobacterium tuberculosis physiologies and antimicrobial susceptibility. PLoS Pathog. 2020 04; 16(4):e1008452. View Abstract
Influence of Plasmodium falciparum Calcium-Dependent Protein Kinase 5 (PfCDPK5) on the Late Schizont Stage Phosphoproteome. mSphere. 2020 01 08; 5(1). View Abstract
Precision Medicine in Pancreatic Disease-Knowledge Gaps and Research Opportunities: Summary of a National Institute of Diabetes and Digestive and Kidney Diseases Workshop. Pancreas. 2019 Nov/Dec; 48(10):1250-1258. View Abstract
Prohibitin is a prognostic marker and therapeutic target to block chemotherapy resistance in Wilms' tumor. JCI Insight. 2019 08 08; 4(15). View Abstract
Dynamic molecular changes during the first week of human life follow a robust developmental trajectory. Nat Commun. 2019 03 12; 10(1):1092. View Abstract
Degradation of the extracellular matrix is part of the pathology of ulcerative colitis. Mol Omics. 2019 02 11; 15(1):67-76. View Abstract
Standard Operating Procedures for Biospecimen Collection, Processing, and Storage: From the Consortium for the Study of Chronic Pancreatitis, Diabetes, and Pancreatic Cancer. Pancreas. 2018 Nov/Dec; 47(10):1213-1221. View Abstract
A Cost-Effective High-Throughput Plasma and Serum Proteomics Workflow Enables Mapping of the Molecular Impact of Total Pancreatectomy with Islet Autotransplantation. J Proteome Res. 2018 05 04; 17(5):1983-1992. View Abstract
Multisystem Analysis of Mycobacterium tuberculosis Reveals Kinase-Dependent Remodeling of the Pathogen-Environment Interface. mBio. 2018 03 06; 9(2). View Abstract
Fetal regional brain protein signature in FASD rat model. Reprod Toxicol. 2018 03; 76:84-92. View Abstract
Erratum: PGBD5 promotes site-specific oncogenic mutations in human tumors. Nat Genet. 2017 09 27; 49(10):1558. View Abstract
Endoscopic Pancreas Fluid Collection: Methods and Relevance for Clinical Care and Translational Science. Am J Gastroenterol. 2016 09; 111(9):1258-66. View Abstract
Proteomics in India: A Report on a Brainstorming Meeting at Hyderabad, India. Mol Cell Proteomics. 2016 07; 15(7):2229-35. View Abstract
f-divergence cutoff index to simultaneously identify differential expression in the integrated transcriptome and proteome. Nucleic Acids Res. 2016 06 02; 44(10):e97. View Abstract
FLEXITau: Quantifying Post-translational Modifications of Tau Protein in Vitro and in Human Disease. Anal Chem. 2016 Apr 05; 88(7):3704-14. View Abstract
Adjuvant-induced Human Monocyte Secretome Profiles Reveal Adjuvant- and Age-specific Protein Signatures. Mol Cell Proteomics. 2016 06; 15(6):1877-94. View Abstract
Progressive Differentiation and Instructive Capacities of Amniotic Fluid and Cerebrospinal Fluid Proteomes following Neural Tube Closure. Dev Cell. 2015 Dec 21; 35(6):789-802. View Abstract
Advancing Urinary Protein Biomarker Discovery by Data-Independent Acquisition on a Quadrupole-Orbitrap Mass Spectrometer. J Proteome Res. 2015 Nov 06; 14(11):4752-62. View Abstract
Characterization of the porcine synovial fluid proteome and a comparison to the plasma proteome. Data Brief. 2015 Dec; 5:241-7. View Abstract
A High-Efficiency Cellular Extraction System for Biological Proteomics. J Proteome Res. 2015 Aug 07; 14(8):3403-8. View Abstract
Zinc regulates a switch between primary and alternative S18 ribosomal proteins in Mycobacterium tuberculosis. Mol Microbiol. 2015 Jul; 97(2):263-80. View Abstract
Spatially heterogeneous choroid plexus transcriptomes encode positional identity and contribute to regional CSF production. J Neurosci. 2015 Mar 25; 35(12):4903-16. View Abstract
A specific LSD1/KDM1A isoform regulates neuronal differentiation through H3K9 demethylation. Mol Cell. 2015 Mar 19; 57(6):957-970. View Abstract
Pancreatic juice prostaglandin e2 concentrations are elevated in chronic pancreatitis and improve detection of early disease. Clin Transl Gastroenterol. 2015 Jan 29; 6:e72. View Abstract
Optimization of cell lysis and protein digestion protocols for protein analysis by LC-MS/MS. Methods Mol Biol. 2015; 1295:259-73. View Abstract
Abundance-based classifier for the prediction of mass spectrometric peptide detectability upon enrichment (PPA). Mol Cell Proteomics. 2015 Feb; 14(2):430-40. View Abstract
Quantitative profiling of peptides from RNAs classified as noncoding. Nat Commun. 2014 Nov 18; 5:5429. View Abstract
An a2-Na/K ATPase/a-adducin complex in astrocytes triggers non-cell autonomous neurodegeneration. Nat Neurosci. 2014 Dec; 17(12):1710-9. View Abstract
A normative study of the synovial fluid proteome from healthy porcine knee joints. J Proteome Res. 2014 Oct 03; 13(10):4377-87. View Abstract
Co-regulation proteomics reveals substrates and mechanisms of APC/C-dependent degradation. EMBO J. 2014 Feb 18; 33(4):385-99. View Abstract
Phenothiazines induce PP2A-mediated apoptosis in T cell acute lymphoblastic leukemia. J Clin Invest. 2014 Feb; 124(2):644-55. View Abstract
The histone demethylase LSD1/KDM1A promotes the DNA damage response. J Cell Biol. 2013 Nov 11; 203(3):457-70. View Abstract
Nonparametric Bayesian evaluation of differential protein quantification. J Proteome Res. 2013 Oct 04; 12(10):4556-65. View Abstract
The XLID protein PQBP1 and the GTPase Dynamin 2 define a signaling link that orchestrates ciliary morphogenesis in postmitotic neurons. Cell Rep. 2013 Sep 12; 4(5):879-89. View Abstract
iFASP: combining isobaric mass tagging with filter-aided sample preparation. J Proteome Res. 2013 Aug 02; 12(8):3809-12. View Abstract
A proteomic comparison of formalin-fixed paraffin-embedded pancreatic tissue from autoimmune pancreatitis, chronic pancreatitis, and pancreatic cancer. JOP. 2013 Jul 10; 14(4):405-14. View Abstract
An in-depth comparison of the male pediatric and adult urinary proteomes. Biochim Biophys Acta. 2014 May; 1844(5):1044-50. View Abstract
Cross-species analysis of nicotine-induced proteomic alterations in pancreatic cells. Proteomics. 2013 May; 13(9):1499-1512. View Abstract
Mass spectrometry-based quantitative proteomic profiling of human pancreatic and hepatic stellate cell lines. Genomics Proteomics Bioinformatics. 2013 Apr; 11(2):105-13. View Abstract
Post-translational modifications of pancreatic fluid proteins collected via the endoscopic pancreatic function test (ePFT). J Proteomics. 2013 Oct 30; 92:216-27. View Abstract
Analysis of endoscopic pancreatic function test (ePFT)-collected pancreatic fluid proteins precipitated via ultracentrifugation. JOP. 2013 Mar 10; 14(2):176-86. View Abstract
Carbamylation of serum albumin as a risk factor for mortality in patients with kidney failure. Sci Transl Med. 2013 Mar 06; 5(175):175ra29. View Abstract
Cytosolic 5'-nucleotidase 1A autoimmunity in sporadic inclusion body myositis. Ann Neurol. 2013 Mar; 73(3):408-18. View Abstract
Gas2l3, a novel constriction site-associated protein whose regulation is mediated by the APC/C Cdh1 complex. PLoS One. 2013; 8(2):e57532. View Abstract
SweetSEQer, simple de novo filtering and annotation of glycoconjugate mass spectra. Mol Cell Proteomics. 2013 Jun; 12(6):1735-40. View Abstract
A non-parametric cutout index for robust evaluation of identified proteins. Mol Cell Proteomics. 2013 Mar; 12(3):807-12. View Abstract
Short Gel, Long Gradient Liquid Chromatography Tandem Mass Spectrometry to Discover Urinary Biomarkers of Chronic Pancreatitis. Open Proteomics J. 2013; 6:1-13. View Abstract
Urine proteomics for discovery of improved diagnostic markers of Kawasaki disease. EMBO Mol Med. 2013 Feb; 5(2):210-20. View Abstract
Post-translational modification: nature's escape from genetic imprisonment and the basis for dynamic information encoding. Wiley Interdiscip Rev Syst Biol Med. 2012 Nov-Dec; 4(6):565-83. View Abstract
A Src family kinase-Shp2 axis controls RUNX1 activity in megakaryocyte and T-lymphocyte differentiation. Genes Dev. 2012 Jul 15; 26(14):1587-601. View Abstract
Integration analysis of quantitative proteomics and transcriptomics data identifies potential targets of frizzled-8 protein-related antiproliferative factor in vivo. BJU Int. 2012 Dec; 110(11 Pt C):E1138-46. View Abstract
Tiki1 is required for head formation via Wnt cleavage-oxidation and inactivation. Cell. 2012 Jun 22; 149(7):1565-77. View Abstract
Mass Spectrometry-Based (GeLC-MS/MS) Comparative Proteomic Analysis of Endoscopically (ePFT) Collected Pancreatic and Gastroduodenal Fluids. Clin Transl Gastroenterol. 2012 May 03; 3:e14. View Abstract
FLEXIQinase, a mass spectrometry-based assay, to unveil multikinase mechanisms. Nat Methods. 2012 Apr 08; 9(5):504-8. View Abstract
Overcoming species boundaries in peptide identification with Bayesian information criterion-driven error-tolerant peptide search (BICEPS). Mol Cell Proteomics. 2012 Jul; 11(7):M111.014167. View Abstract
Mass spectrometry-based proteomics for translational research: a technical overview. Yale J Biol Med. 2012 Mar; 85(1):59-73. View Abstract
Proteomic analysis of formalin-fixed paraffin-embedded pancreatic tissue using liquid chromatography tandem mass spectrometry. Pancreas. 2012 Mar; 41(2):175-85. View Abstract
Proteomic analysis (GeLC-MS/MS) of ePFT-collected pancreatic fluid in chronic pancreatitis. J Proteome Res. 2012 Mar 02; 11(3):1897-912. View Abstract
Detection and diagnostic value of urine leucine-rich a-2-glycoprotein in children with suspected acute appendicitis. Ann Emerg Med. 2012 Jul; 60(1):78-83.e1. View Abstract
Etiology of limb girdle muscular dystrophy 1D/1E determined by laser capture microdissection proteomics. Ann Neurol. 2012 Jan; 71(1):141-5. View Abstract
A practical guide to the FLEXIQuant method. Methods Mol Biol. 2012; 893:295-319. View Abstract
Optimization of cell lysis and protein digestion protocols for the analysis of HeLa S3 cells by LC-MS/MS. Proteomics. 2011 Dec; 11(24):4726-30. View Abstract
Design and validation of a high-throughput matrix-assisted laser desorption ionization time-of-flight mass spectrometry method for quantification of hepcidin in human plasma. Anal Chem. 2011 Nov 01; 83(21):8357-62. View Abstract
Brainstem deficiency of the 14-3-3 regulator of serotonin synthesis: a proteomics analysis in the sudden infant death syndrome. Mol Cell Proteomics. 2012 Jan; 11(1):M111.009530. View Abstract
Life cycle stage-resolved proteomic analysis of the excretome/secretome from Strongyloides ratti--identification of stage-specific proteases. Mol Cell Proteomics. 2011 Dec; 10(12):M111.010157. View Abstract
mz5: space- and time-efficient storage of mass spectrometry data sets. Mol Cell Proteomics. 2012 Jan; 11(1):O111.011379. View Abstract
Proteomic analysis of a rat pancreatic stellate cell line using liquid chromatography tandem mass spectrometry (LC-MS/MS). J Proteomics. 2011 Dec 21; 75(2):708-17. View Abstract
A polymorphism in the leptin gene promoter is associated with anemia in patients with HIV disease. Blood. 2011 Nov 17; 118(20):5401-8. View Abstract
Proteomic analysis of an immortalized mouse pancreatic stellate cell line identifies differentially-expressed proteins in activated vs nonproliferating cell states. J Proteome Res. 2011 Oct 07; 10(10):4835-44. View Abstract
Stage-specific excretory-secretory small heat shock proteins from the parasitic nematode Strongyloides ratti--putative links to host's intestinal mucosal defense system. FEBS J. 2011 Sep; 278(18):3319-36. View Abstract
Proteome of human perilymph. J Proteome Res. 2011 Sep 02; 10(9):3845-51. View Abstract
Difference gel electrophoresis identifies differentially expressed proteins in endoscopically collected pancreatic fluid. Electrophoresis. 2011 Aug; 32(15):1939-51. View Abstract
Cytokine profiling of pancreatic fluid using the ePFT collection method in tandem with a multiplexed microarray assay. J Immunol Methods. 2011 Jun 30; 369(1-2):98-107. View Abstract
Comparative analysis of Erk phosphorylation suggests a mixed strategy for measuring phospho-form distributions. Mol Syst Biol. 2011 Apr 12; 7:482. View Abstract
Quantitative proteomics identifies a beta-catenin network as an element of the signaling response to Frizzled-8 protein-related antiproliferative factor. Mol Cell Proteomics. 2011 Jun; 10(6):M110.007492. View Abstract
Mass spectrometry-based proteomics of endoscopically collected pancreatic fluid in chronic pancreatitis research. Proteomics Clin Appl. 2011 Apr; 5(3-4):109-20. View Abstract
libfbi: a C++ implementation for fast box intersection and application to sparse mass spectrometry data. Bioinformatics. 2011 Apr 15; 27(8):1166-7. View Abstract
One-step sample concentration, purification, and albumin depletion method for urinary proteomics. J Proteome Res. 2010 Nov 05; 9(11):6082-9. View Abstract
Iron overload in patients with acute leukemia or MDS undergoing myeloablative stem cell transplantation. Biol Blood Marrow Transplant. 2011 Jun; 17(6):852-60. View Abstract
Proteomic analysis of endoscopically (endoscopic pancreatic function test) collected gastroduodenal fluid using in-gel tryptic digestion followed by LC-MS/MS. Proteomics Clin Appl. 2010 Sep; 4(8-9):715-25. View Abstract
Identification of pancreas-specific proteins in endoscopically (endoscopic pancreatic function test) collected pancreatic fluid with liquid chromatography--tandem mass spectrometry. Pancreas. 2010 Aug; 39(6):889-96. View Abstract
Optimized sample preparation of endoscopic collected pancreatic fluid for SDS-PAGE analysis. Electrophoresis. 2010 Jul; 31(14):2377-87. View Abstract
Quantitative proteomics analysis reveals molecular networks regulated by epidermal growth factor receptor level in head and neck cancer. J Proteome Res. 2010 Jun 04; 9(6):3073-82. View Abstract
Estimating the confidence of peptide identifications without decoy databases. Anal Chem. 2010 Jun 01; 82(11):4314-8. View Abstract
Effect of high-accuracy precursor masses on phosphopeptide identification from MS3 spectra. Anal Chem. 2010 May 15; 82(10):3977-80. View Abstract
MGFp: an open Mascot Generic Format parser library implementation. J Proteome Res. 2010 May 07; 9(5):2762-3. View Abstract
Deuteration distribution estimation with improved sequence coverage for HX/MS experiments. Bioinformatics. 2010 Jun 15; 26(12):1535-41. View Abstract
Extensive phosphorylation with overlapping specificity by Mycobacterium tuberculosis serine/threonine protein kinases. Proc Natl Acad Sci U S A. 2010 Apr 20; 107(16):7521-6. View Abstract
High-throughput matrix-assisted laser desorption ionization-time-of-flight mass spectrometry method for quantification of hepcidin in human urine. Anal Chem. 2010 Feb 15; 82(4):1551-5. View Abstract
Non-linear classification for on-the-fly fractional mass filtering and targeted precursor fragmentation in mass spectrometry experiments. Bioinformatics. 2010 Mar 15; 26(6):791-7. View Abstract
When less can yield more - Computational preprocessing of MS/MS spectra for peptide identification. Proteomics. 2009 Nov; 9(21):4978-84. View Abstract
Computational protein profile similarity screening for quantitative mass spectrometry experiments. Bioinformatics. 2010 Jan 01; 26(1):77-83. View Abstract
Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes. Mol Cell Proteomics. 2010 Jan; 9(1):54-70. View Abstract
Binding partner switching on microtubules and aurora-B in the mitosis to cytokinesis transition. Mol Cell Proteomics. 2010 Feb; 9(2):336-50. View Abstract
Urine proteomics for profiling of human disease using high accuracy mass spectrometry. Proteomics Clin Appl. 2009 Sep 01; 3(9):1052-1061. View Abstract
Molecular and functional characterisation of the heat shock protein 10 of Strongyloides ratti. Mol Biochem Parasitol. 2009 Dec; 168(2):149-57. View Abstract
Proteomics and opportunities for clinical translation in urological disease. J Urol. 2009 Sep; 182(3):835-43. View Abstract
Discovery and validation of urine markers of acute pediatric appendicitis using high-accuracy mass spectrometry. Ann Emerg Med. 2010 Jan; 55(1):62-70.e4. View Abstract
Differentiation-dependent interactions between RUNX-1 and FLI-1 during megakaryocyte development. Mol Cell Biol. 2009 Aug; 29(15):4103-15. View Abstract
FLEXIQuant: a novel tool for the absolute quantification of proteins, and the simultaneous identification and quantification of potentially modified peptides. J Proteome Res. 2009 May; 8(5):2201-10. View Abstract
The mechanism and pattern of yolk consumption provide insight into embryonic nutrition in Xenopus. Development. 2009 May; 136(9):1539-48. View Abstract
A simple and effective method for detecting phosphopeptides for phosphoproteomic analysis. J Proteomics. 2009 Jul 21; 72(5):831-5. View Abstract
Urine proteomics for profiling of human disease using high accuracy mass spectrometry. Proteomics Clin Appl. 2009; 3:1052-61. View Abstract
NITPICK: peak identification for mass spectrometry data. BMC Bioinformatics. 2008 Aug 28; 9:355. View Abstract
Robust prediction of the MASCOT score for an improved quality assessment in mass spectrometric proteomics. J Proteome Res. 2008 Sep; 7(9):3708-17. View Abstract
Rapid preparation of nuclei-depleted detergent-resistant membrane fractions suitable for proteomics analysis. BMC Cell Biol. 2008 Jun 05; 9:30. View Abstract
Alkaline Phosphatase Treatment of Phosphopeptides: In-Solution Dephosphorylation prior to MALDI-MS Analysis. CSH Protoc. 2008 May 01; 2008:pdb.prot4610. View Abstract
Alkaline Phosphatase Treatment of Phosphopeptides: In-Solution Dephosphorylation after MALDI-MS Analysis. CSH Protoc. 2008 May 01; 2008:pdb.prot4611. View Abstract
Alkaline Phosphatase Treatment of Phosphopeptides: On-Probe Dephosphorylation after MALDI-MS Analysis. CSH Protoc. 2008 May 01; 2008:pdb.prot4612. View Abstract
Different phosphorylation states of the anaphase promoting complex in response to antimitotic drugs: a quantitative proteomic analysis. Proc Natl Acad Sci U S A. 2008 Apr 22; 105(16):6069-74. View Abstract
SALAMI (Spectrum ALignments using high Accuracy Mass and hIgh sensitivity data): how to make the best out of hybrid MS/MS data. J Proteome Res. 2008 May; 7(5):1984-93. View Abstract
Temporal variations of the postnatal rat urinary proteome as a reflection of systemic maturation. Proteomics. 2008 Mar; 8(5):1097-112. View Abstract
Proteomic approaches to the analysis of multiprotein signaling complexes. Proteomics. 2008 Feb; 8(4):832-51. View Abstract
Phosphopeptide Purification by IMAC with Fe(III) and Ga(III). CSH Protoc. 2007 Nov 01; 2007:pdb.prot4607. View Abstract
Preparation and Use of Microcolumns for Sample Desalting or Nanoscale IMAC. CSH Protoc. 2007 Nov 01; 2007:pdb.prot4608. View Abstract
amsrpm: Robust Point Matching for Retention Time Alignment of LC/MS Data with R. J Stat Software. 2007; 18:4. View Abstract
Why b, y’s? Sodiation-induced tryptic peptide-like fragmentation of non-tryptic peptides. Int J Mass Spectrom. 2007; 268:181-189. View Abstract
A quantitative proteomic analysis of growth factor-induced compositional changes in lipid rafts of human smooth muscle cells. Proteomics. 2005 Dec; 5(18):4733-42. View Abstract
Phosphorylation analysis by mass spectrometry: myths, facts, and the consequences for qualitative and quantitative measurements. Mol Cell Proteomics. 2006 Jan; 5(1):172-81. View Abstract
Stable isotope-free relative and absolute quantitation of protein phosphorylation stoichiometry by MS. Proc Natl Acad Sci U S A. 2005 Mar 15; 102(11):3948-53. View Abstract
Purification of active TFIID from Saccharomyces cerevisiae. Extensive promoter contacts and co-activator function. J Biol Chem. 2004 Nov 26; 279(48):49973-81. View Abstract
The ABC's (and XYZ's) of peptide sequencing. Nat Rev Mol Cell Biol. 2004 Sep; 5(9):699-711. View Abstract
Autophosphorylation of JAK2 on tyrosines 221 and 570 regulates its activity. Mol Cell Biol. 2004 Jun; 24(11):4955-67. View Abstract
Purification and architecture of the ubiquitous Wave complex. Proc Natl Acad Sci U S A. 2004 Mar 30; 101(13):4379-83. View Abstract
Development of human protein reference database as an initial platform for approaching systems biology in humans. Genome Res. 2003 Oct; 13(10):2363-71. View Abstract
Protein profiling with cleavable isotope-coded affinity tag (cICAT) reagents: the yeast salinity stress response. Mol Cell Proteomics. 2003 Nov; 2(11):1198-204. View Abstract
Using optimized collision energies and high resolution, high accuracy fragment ion selection to improve glycopeptide detection by precursor ion scanning. J Am Soc Mass Spectrom. 2003 Jul; 14(7):777-84. View Abstract
Phosphotyrosine mapping in Bcr/Abl oncoprotein using phosphotyrosine-specific immonium ion scanning. Mol Cell Proteomics. 2003 Mar; 2(3):138-45. View Abstract
Analysis of bromotryptophan and hydroxyproline modifications by high-resolution, high-accuracy precursor ion scanning utilizing fragment ions with mass-deficient mass tags. Anal Chem. 2002 Dec 15; 74(24):6230-6. View Abstract
Analysis of tyrosine phosphorylation sites in signaling molecules by a phosphotyrosine-specific immonium ion scanning method. Sci STKE. 2002 Oct 15; 2002(154):pl16. View Abstract
A new derivatization strategy for the analysis of phosphopeptides by precursor ion scanning in positive ion mode. J Am Soc Mass Spectrom. 2002 Aug; 13(8):996-1003. View Abstract
Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome. Trends Biotechnol. 2002 Jun; 20(6):261-8. View Abstract
Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. Mol Cell Proteomics. 2002 May; 1(5):376-86. View Abstract
Analysis of protein-nucleic acid interactions by photochemical cross-linking and mass spectrometry. Mass Spectrom Rev. 2002 May-Jun; 21(3):163-82. View Abstract
Multi-Protein Complexes Studied by Mass Spectrometry. ScientificWorldJournal. 2002; 2:91-92. View Abstract
Directed proteomic analysis of the human nucleolus. Curr Biol. 2002 Jan 08; 12(1):1-11. View Abstract
Loss of chromosome 11q21-23.1 and 17p and gain of chromosome 6p are independent prognostic indicators in B-cell non-Hodgkin's lymphoma. Br J Cancer. 2001 Dec 14; 85(12):1900-13. View Abstract
GPMAW--a software tool for analyzing proteins and peptides. Trends Biochem Sci. 2001 Nov; 26(11):687-9. View Abstract
Tyrosine phosphorylation mapping of the epidermal growth factor receptor signaling pathway. J Biol Chem. 2002 Jan 11; 277(2):1031-9. View Abstract
Mass spectrometric analysis of a UV-cross-linked protein-DNA complex: tryptophans 54 and 88 of E. coli SSB cross-link to DNA. Protein Sci. 2001 Oct; 10(10):1989-2001. View Abstract
Quadrupole time-of-flight versus triple-quadrupole mass spectrometry for the determination of phosphopeptides by precursor ion scanning. J Mass Spectrom. 2001 Jul; 36(7):782-90. View Abstract
Labile sulfogroup allows differentiation of sulfotyrosine and phosphotyrosine in peptides. J Mass Spectrom. 2001 Jul; 36(7):832-3. View Abstract
Investigation of tyrosine nitration in proteins by mass spectrometry. J Mass Spectrom. 2001 Jun; 36(6):616-25. View Abstract
Detection of tyrosine phosphorylated peptides by precursor ion scanning quadrupole TOF mass spectrometry in positive ion mode. Anal Chem. 2001 Apr 01; 73(7):1440-8. View Abstract
Molecular patterning of the oikoplastic epithelium of the larvacean tunicate Oikopleura dioica. J Biol Chem. 2001 Jun 08; 276(23):20624-32. View Abstract
Similarity between condensed phase and gas phase chemistry: fragmentation of peptides containing oxidized cysteine residues and its implications for proteomics. J Am Soc Mass Spectrom. 2001 Feb; 12(2):228-32. View Abstract
Mass Spectrometry for the Analysis of Protein Modifications (Ph.D. Thesis). 2001. View Abstract
Identification of a novel immunoreceptor tyrosine-based activation motif-containing molecule, STAM2, by mass spectrometry and its involvement in growth factor and cytokine receptor signaling pathways. J Biol Chem. 2000 Dec 08; 275(49):38633-9. View Abstract
Gap junctional intercellular communication is not a major mediator in the bystander effect in photodynamic treatment of MDCK II cells. Radiat Res. 2000 Sep; 154(3):331-41. View Abstract
The bystander effect in photodynamic inactivation of cells. Biochim Biophys Acta. 2000 Jul 26; 1475(3):273-80. View Abstract
Oncogenic aberrations in the p53 pathway are associated with a high S phase fraction and poor patient survival in B-cell Non-Hodgkin's lymphoma. Int J Cancer. 2000 Jul 20; 89(4):313-24. View Abstract
Detection and quantification of neurotensin in human brain tissue by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Chem. 2000 Jul 15; 72(14):3320-6. View Abstract
Phenyl thiocarbamoyl and related derivatives of peptides: Edman chemistry in the gas phase. Int J Mass Spectrom. 1999; 188:95-103. View Abstract
The SHi Reaction at Silicon – A New Entry into Cyclic Alkoxy-silanes. Chem Eur J. 1999; 5:759-73. View Abstract
Stereoselective Phenyl Migration from Silicon to Carbon. Tetrahedron Lett. 1998; 39:8829-32. View Abstract
Intramolecular Homolytic Substitution on Silicon – Influence of the Degree of Substitution on the Rate of Cyclisation (Dipl. Chem. Thesis). 1998. View Abstract
Techniques for the Mass Spectrometric Localisation of Double Bonds (B.Sc. Thesis). 1995. View Abstract
The initiation mass for DNA replication in Escherichia coli K-12 is dependent on growth rate. EMBO J. 1994 May 01; 13(9):2097-102. View Abstract
Characterization of singlet oxygen-induced guanine residue damage after photochemical treatment of free nucleosides and DNA. Biochim Biophys Acta. 1994 Jan 18; 1217(1):9-15. View Abstract
Plateau distributions of DNA fragment lengths produced by extended light exposure of extranuclear photosensitizers in human cells. Nucleic Acids Res. 1992 Dec 25; 20(24):6687-93. View Abstract
Differential chromatin structure-dependent binding of 7-aminoactinomycin D in normal and malignant bone marrow hematopoietic cells. Cancer Res. 1992 Sep 15; 52(18):5007-12. View Abstract
Synergistic effects of photoactivated tetra(4-sulfonatophenyl)porphine and nocodazole on microtubule assembly, accumulation of cells in mitosis and cell survival. J Photochem Photobiol B. 1992 Apr 15; 13(1):59-70. View Abstract
Cell cycle-specific expression and nuclear binding of DNA polymerase alpha. Mol Cell Biol. 1991 Jun; 11(6):3384-9. View Abstract
Fluorescence spectra of Hoechst 33258 bound to chromatin. Biochim Biophys Acta. 1986 Oct 16; 868(1):17-23. View Abstract
Timing of initiation of chromosome replication in individual Escherichia coli cells. EMBO J. 1986 Jul; 5(7):1711-7. View Abstract
Neither adriamycin nor actinomycin D displaces Tb3+ from DNA. Biochim Biophys Acta. 1985 Aug 21; 825(4):416-8. View Abstract
Promotive and inhibitory effects of macrophages on the mitogen-induced blastogenesis of lymphocytes: a flow-cytometric study. Scand J Immunol. 1981 Jul; 14(1):39-46. View Abstract
Further developments of a microscope-based flow cytometer: light scatter detection and excitation intensity compensation. Cytometry. 1980 Jul; 1(1):26-31. View Abstract
Molecular excitations in radiation damage as studies by the radiation-induced luminescence of proteins. Scand J Clin Lab Invest Suppl. 1968; 106:21-30. View Abstract