Research Overview

Hanno Steen is a pioneer in developing methods for the qualitative and quantitative analysis of protein modifications by mass spectrometry, with special emphasis on tyrosine phosphorylation. In carcinogenesis, the pivotal role of tyrosine phosphorylation is underscored by the notion that almost half of the oncogenes known to date encode tyrosine kinases.

Hanno Steen's interests are focused on proteomics and biological mass spectrometry in general. He is particularly interested in the mass spectrometric analysis of the composition of complex protein mixtures, which includes not only the cataloging but also the comprehensive characterization of the components.

Dr. Steen's lab is working on novel methods for identifying and quantifying various protein modifications that are involved in pediatric diseases in order to provide new insights into the underlying biological processes, a prerequisite for a rational approach to prevent and/or cure these diseases.

Another goal of the lab's research is the development and applications of screening methods to detect and identify diagnostic and prognostic disease markers in body fluids, which offer an easily accessible mirror of the state of the entire body. These biomarkers can guide treatment decisions, predict patient phenotypes, and allow for early diagnosis.

Research Background

Dr. Steen studied chemistry at the University of Freiburg (Germany), UMIST/Manchester (UK), and Swiss Federal Institute of Technology (ETH) Zurich (Switzerland). He received his PhD in Biochemistry and Molecular Biology from the University of Southern Denmark, working in the laboratory of Dr. Matthias Mann on the mass spectrometric analysis of protein modifications. He completed postdoctoral fellowships in the laboratories of Drs. Steve Gygi and Marc Kirschner in the Departments of Cell Biology and Systems Biology at Harvard Medical School.

In 2004, Dr. Steen joined the faculty at Harvard Medical School and became principal investigator in the Department of Pathology at Boston Children's Hospital. Dr. Steen has published more than 180 papers in international peer-reviewed journals, which have been cited more than 23,000 times, resulting in an h-index of 66.

Publications

Publications

  1. Dementia with lewy bodies patients with high tau levels display unique proteome profiles. Mol Neurodegener. 2024 Dec 19; 19(1):98. View Abstract
  2. Chronic Viral Reactivation and Associated Host Immune Response and Clinical Outcomes in Acute COVID-19 and Post-Acute Sequelae of COVID-19. bioRxiv. 2024 Nov 16. View Abstract
  3. Predictive gene expression signature diagnoses neonatal sepsis before clinical presentation. EBioMedicine. 2024 Dec; 110:105411. View Abstract
  4. The BNT162b2 mRNA vaccine demonstrates reduced age-associated TH1 support in vitro and in vivo. iScience. 2024 Nov 15; 27(11):111055. View Abstract
  5. Plasma adenosine deaminase-1 and -2 activities are lower at birth in Papua New Guinea than in The Gambia but converge over the first weeks of life. Front Immunol. 2024; 15:1425349. View Abstract
  6. PEPerMINT: peptide abundance imputation in mass spectrometry-based proteomics using graph neural networks. Bioinformatics. 2024 09 01; 40(Suppl 2):ii70-ii78. View Abstract
  7. Longitudinal plasma proteomic analysis of 1117 hospitalized patients with COVID-19 identifies features associated with severity and outcomes. Sci Adv. 2024 May 24; 10(21):eadl5762. View Abstract
  8. Integrated longitudinal multiomics study identifies immune programs associated with acute COVID-19 severity and mortality. J Clin Invest. 2024 May 01; 134(9). View Abstract
  9. Host-microbe multiomic profiling reveals age-dependent immune dysregulation associated with COVID-19 immunopathology. Sci Transl Med. 2024 Apr 17; 16(743):eadj5154. View Abstract
  10. Diagnostic and Prognostic Biomarkers of Chronic Pancreatitis: A Conceptual Framework Based on the PRoBE Design. Gastroenterology. 2024 06; 166(6):957-962.e3. View Abstract
  11. Quantitative profiling of posttranslational modifications of pathological tau via sarkosyl fractionation and mass spectrometry. Nat Protoc. 2024 Apr; 19(4):1235-1251. View Abstract
  12. Analytical challenges in omics research on asthma and allergy: A National Institute of Allergy and Infectious Diseases workshop. J Allergy Clin Immunol. 2024 Apr; 153(4):954-968. View Abstract
  13. Features of acute COVID-19 associated with post-acute sequelae of SARS-CoV-2 phenotypes: results from the IMPACC study. Nat Commun. 2024 Jan 03; 15(1):216. View Abstract
  14. Integrated longitudinal multi-omics study identifies immune programs associated with COVID-19 severity and mortality in 1152 hospitalized participants. bioRxiv. 2023 Nov 06. View Abstract
  15. Early cellular and molecular signatures correlate with severity of West Nile virus infection. iScience. 2023 Dec 15; 26(12):108387. View Abstract
  16. Proteomics-Based Mapping of Bronchopulmonary Dysplasia-Associated Changes in Noninvasively Accessible Oral Secretions. J Pediatr. 2024 Jul; 270:113774. View Abstract
  17. Multi-omic longitudinal study reveals immune correlates of clinical course among hospitalized COVID-19 patients. Cell Rep Med. 2023 06 20; 4(6):101079. View Abstract
  18. Plasma proteome perturbation for CMV DNAemia in kidney transplantation. PLoS One. 2023; 18(5):e0285870. View Abstract
  19. Integrative systems biology characterizes immune-mediated neurodevelopmental changes in murine Zika virus microcephaly. iScience. 2023 Jul 21; 26(7):106909. View Abstract
  20. Meta-analysis of published cerebrospinal fluid proteomics data identifies and validates metabolic enzyme panel as Alzheimer's disease biomarkers. Cell Rep Med. 2023 04 18; 4(4):101005. View Abstract
  21. A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics. Sci Adv. 2023 03 29; 9(13):eadf9717. View Abstract
  22. Choroid plexus-targeted NKCC1 overexpression to treat post-hemorrhagic hydrocephalus. Neuron. 2023 05 17; 111(10):1591-1608.e4. View Abstract
  23. Common mouse models of tauopathy reflect early but not late human disease. Mol Neurodegener. 2023 02 02; 18(1):10. View Abstract
  24. The mRNA vaccine BNT162b2 demonstrates impaired TH1 immunogenicity in human elders in vitro and aged mice in vivo. Res Sq. 2022 Dec 21. View Abstract
  25. Using CSF Proteomics to Investigate Herpesvirus Infections of the Central Nervous System. Viruses. 2022 12 10; 14(12). View Abstract
  26. Longitudinal serum proteomics analyses identify unique and overlapping host response pathways in Lyme disease and West Nile virus infection. Front Immunol. 2022; 13:1012824. View Abstract
  27. A Parallelization Strategy for the Time Efficient Analysis of Thousands of LC/MS Runs in High-Performance Computing Environment. J Proteome Res. 2022 11 04; 21(11):2810-2814. View Abstract
  28. CAF08 adjuvant enables single dose protection against respiratory syncytial virus infection in murine newborns. Nat Commun. 2022 08 02; 13(1):4234. View Abstract
  29. Using plasma proteomics to investigate viral infections of the central nervous system including patients with HIV-associated neurocognitive disorders. J Neurovirol. 2022 06; 28(3):341-354. View Abstract
  30. Bacille Calmette-Guérin vaccine reprograms human neonatal lipid metabolism in vivo and in vitro. Cell Rep. 2022 05 03; 39(5):110772. View Abstract
  31. Urine Proteomics Reveals Sex-Specific Response to Total Pancreatectomy With Islet Autotransplantation. Pancreas. 2022 05 01; 51(5):435-444. View Abstract
  32. Plasma Proteomic Analysis Distinguishes Severity Outcomes of Human Ebola Virus Disease. mBio. 2022 06 28; 13(3):e0056722. View Abstract
  33. Determinants of B-Cell Compartment Hyperactivation in European Adolescents Living With Perinatally Acquired HIV-1 After Over 10 Years of Suppressive Therapy. Front Immunol. 2022; 13:860418. View Abstract
  34. multiFLEX-LF: A Computational Approach to Quantify the Modification Stoichiometries in Label-Free Proteomics Data Sets. J Proteome Res. 2022 04 01; 21(4):899-909. View Abstract
  35. Urine Proteomics for Noninvasive Monitoring of Biomarkers in Bronchopulmonary Dysplasia. Neonatology. 2022; 119(2):193-203. View Abstract
  36. Proteomics based markers of clinical pain severity in juvenile idiopathic arthritis. Pediatr Rheumatol Online J. 2022 Jan 15; 20(1):3. View Abstract
  37. Using 'Big Data' to Disentangle Aging and COVID-19. Nat Aging. 2021 06; 1(6):496-497. View Abstract
  38. Multi-omic regulatory networks capture downstream effects of kinase inhibition in Mycobacterium tuberculosis. NPJ Syst Biol Appl. 2021 01 29; 7(1):8. View Abstract
  39. Biomarkers of Chronic Pancreatitis: A systematic literature review. Pancreatology. 2021 Mar; 21(2):323-333. View Abstract
  40. Sample Preparation for High-Throughput Urine Proteomics Using 96-Well Polyvinylidene Fluoride (PVDF) Membranes. Adv Exp Med Biol. 2021; 1306:1-12. View Abstract
  41. Delayed Processing of Secretin-Induced Pancreas Fluid Influences the Quality and Integrity of Proteins and Nucleic Acids. Pancreas. 2021 01 01; 50(1):17-28. View Abstract
  42. FLEXIQuant-LF to quantify protein modification extent in label-free proteomics data. Elife. 2020 12 07; 9. View Abstract
  43. Corrigendum: Clinical Protocol for a Longitudinal Cohort Study Employing Systems Biology to Identify Markers of Vaccine Immunogenicity in Newborn Infants in The Gambia and Papua New Guinea. Front Pediatr. 2020; 8:610461. View Abstract
  44. Tau PTM Profiles Identify Patient Heterogeneity and Stages of Alzheimer's Disease. Cell. 2020 12 10; 183(6):1699-1713.e13. View Abstract
  45. Author Correction: PGBD5 promotes site-specific oncogenic mutations in human tumors. Nat Genet. 2020 Nov; 52(11):1265. View Abstract
  46. Preparing for Life: Plasma Proteome Changes and Immune System Development During the First Week of Human Life. Front Immunol. 2020; 11:578505. View Abstract
  47. Antioxidant functions of DHHC3 suppress anti-cancer drug activities. Cell Mol Life Sci. 2021 Mar; 78(5):2341-2353. View Abstract
  48. Phosphorylation of ACTN4 Leads to Podocyte Vulnerability and Proteinuric Glomerulosclerosis. J Am Soc Nephrol. 2020 07; 31(7):1479-1495. View Abstract
  49. Clinical Protocol for a Longitudinal Cohort Study Employing Systems Biology to Identify Markers of Vaccine Immunogenicity in Newborn Infants in The Gambia and Papua New Guinea. Front Pediatr. 2020; 8:197. View Abstract
  50. Protein kinases PknA and PknB independently and coordinately regulate essential Mycobacterium tuberculosis physiologies and antimicrobial susceptibility. PLoS Pathog. 2020 04; 16(4):e1008452. View Abstract
  51. Influence of Plasmodium falciparum Calcium-Dependent Protein Kinase 5 (PfCDPK5) on the Late Schizont Stage Phosphoproteome. mSphere. 2020 01 08; 5(1). View Abstract
  52. Precision Medicine in Pancreatic Disease-Knowledge Gaps and Research Opportunities: Summary of a National Institute of Diabetes and Digestive and Kidney Diseases Workshop. Pancreas. 2019 Nov/Dec; 48(10):1250-1258. View Abstract
  53. Prohibitin is a prognostic marker and therapeutic target to block chemotherapy resistance in Wilms' tumor. JCI Insight. 2019 08 08; 4(15). View Abstract
  54. Dynamic molecular changes during the first week of human life follow a robust developmental trajectory. Nat Commun. 2019 03 12; 10(1):1092. View Abstract
  55. Degradation of the extracellular matrix is part of the pathology of ulcerative colitis. Mol Omics. 2019 02 11; 15(1):67-76. View Abstract
  56. Standard Operating Procedures for Biospecimen Collection, Processing, and Storage: From the Consortium for the Study of Chronic Pancreatitis, Diabetes, and Pancreatic Cancer. Pancreas. 2018 Nov/Dec; 47(10):1213-1221. View Abstract
  57. A Cost-Effective High-Throughput Plasma and Serum Proteomics Workflow Enables Mapping of the Molecular Impact of Total Pancreatectomy with Islet Autotransplantation. J Proteome Res. 2018 05 04; 17(5):1983-1992. View Abstract
  58. Multisystem Analysis of Mycobacterium tuberculosis Reveals Kinase-Dependent Remodeling of the Pathogen-Environment Interface. mBio. 2018 03 06; 9(2). View Abstract
  59. Fetal regional brain protein signature in FASD rat model. Reprod Toxicol. 2018 03; 76:84-92. View Abstract
  60. Erratum: PGBD5 promotes site-specific oncogenic mutations in human tumors. Nat Genet. 2017 09 27; 49(10):1558. View Abstract
  61. Placental Proteomics Reveal Insights into Fetal Alcohol Spectrum Disorders. Alcohol Clin Exp Res. 2017 Sep; 41(9):1551-1558. View Abstract
  62. PIQED: automated identification and quantification of protein modifications from DIA-MS data. Nat Methods. 2017 06 29; 14(7):646-647. View Abstract
  63. PGBD5 promotes site-specific oncogenic mutations in human tumors. Nat Genet. 2017 Jul; 49(7):1005-1014. View Abstract
  64. High-Throughput Parallel Proteomic Sample Preparation Using 96-Well Polyvinylidene Fluoride (PVDF) Membranes and C18 Purification Plates. Methods Mol Biol. 2017; 1619:395-402. View Abstract
  65. Endoscopic Pancreas Fluid Collection: Methods and Relevance for Clinical Care and Translational Science. Am J Gastroenterol. 2016 09; 111(9):1258-66. View Abstract
  66. Urinary Proteomics Yield Pathological Insights for Ureteropelvic Junction Obstruction. Mol Cell Proteomics. 2016 08; 15(8):2607-15. View Abstract
  67. Proteomics in India: A Report on a Brainstorming Meeting at Hyderabad, India. Mol Cell Proteomics. 2016 07; 15(7):2229-35. View Abstract
  68. f-divergence cutoff index to simultaneously identify differential expression in the integrated transcriptome and proteome. Nucleic Acids Res. 2016 06 02; 44(10):e97. View Abstract
  69. FLEXITau: Quantifying Post-translational Modifications of Tau Protein in Vitro and in Human Disease. Anal Chem. 2016 Apr 05; 88(7):3704-14. View Abstract
  70. Adjuvant-induced Human Monocyte Secretome Profiles Reveal Adjuvant- and Age-specific Protein Signatures. Mol Cell Proteomics. 2016 06; 15(6):1877-94. View Abstract
  71. Progressive Differentiation and Instructive Capacities of Amniotic Fluid and Cerebrospinal Fluid Proteomes following Neural Tube Closure. Dev Cell. 2015 Dec 21; 35(6):789-802. View Abstract
  72. Advancing Urinary Protein Biomarker Discovery by Data-Independent Acquisition on a Quadrupole-Orbitrap Mass Spectrometer. J Proteome Res. 2015 Nov 06; 14(11):4752-62. View Abstract
  73. Characterization of the porcine synovial fluid proteome and a comparison to the plasma proteome. Data Brief. 2015 Dec; 5:241-7. View Abstract
  74. MStern Blotting-High Throughput Polyvinylidene Fluoride (PVDF) Membrane-Based Proteomic Sample Preparation for 96-Well Plates. Mol Cell Proteomics. 2015 Oct; 14(10):2814-23. View Abstract
  75. A High-Efficiency Cellular Extraction System for Biological Proteomics. J Proteome Res. 2015 Aug 07; 14(8):3403-8. View Abstract
  76. Zinc regulates a switch between primary and alternative S18 ribosomal proteins in Mycobacterium tuberculosis. Mol Microbiol. 2015 Jul; 97(2):263-80. View Abstract
  77. Spatially heterogeneous choroid plexus transcriptomes encode positional identity and contribute to regional CSF production. J Neurosci. 2015 Mar 25; 35(12):4903-16. View Abstract
  78. A specific LSD1/KDM1A isoform regulates neuronal differentiation through H3K9 demethylation. Mol Cell. 2015 Mar 19; 57(6):957-970. View Abstract
  79. Pancreatic juice prostaglandin e2 concentrations are elevated in chronic pancreatitis and improve detection of early disease. Clin Transl Gastroenterol. 2015 Jan 29; 6:e72. View Abstract
  80. Optimization of cell lysis and protein digestion protocols for protein analysis by LC-MS/MS. Methods Mol Biol. 2015; 1295:259-73. View Abstract
  81. Abundance-based classifier for the prediction of mass spectrometric peptide detectability upon enrichment (PPA). Mol Cell Proteomics. 2015 Feb; 14(2):430-40. View Abstract
  82. Quantitative profiling of peptides from RNAs classified as noncoding. Nat Commun. 2014 Nov 18; 5:5429. View Abstract
  83. An a2-Na/K ATPase/a-adducin complex in astrocytes triggers non-cell autonomous neurodegeneration. Nat Neurosci. 2014 Dec; 17(12):1710-9. View Abstract
  84. A normative study of the synovial fluid proteome from healthy porcine knee joints. J Proteome Res. 2014 Oct 03; 13(10):4377-87. View Abstract
  85. Co-regulation proteomics reveals substrates and mechanisms of APC/C-dependent degradation. EMBO J. 2014 Feb 18; 33(4):385-99. View Abstract
  86. Phenothiazines induce PP2A-mediated apoptosis in T cell acute lymphoblastic leukemia. J Clin Invest. 2014 Feb; 124(2):644-55. View Abstract
  87. The histone demethylase LSD1/KDM1A promotes the DNA damage response. J Cell Biol. 2013 Nov 11; 203(3):457-70. View Abstract
  88. Nonparametric Bayesian evaluation of differential protein quantification. J Proteome Res. 2013 Oct 04; 12(10):4556-65. View Abstract
  89. The XLID protein PQBP1 and the GTPase Dynamin 2 define a signaling link that orchestrates ciliary morphogenesis in postmitotic neurons. Cell Rep. 2013 Sep 12; 4(5):879-89. View Abstract
  90. iFASP: combining isobaric mass tagging with filter-aided sample preparation. J Proteome Res. 2013 Aug 02; 12(8):3809-12. View Abstract
  91. A proteomic comparison of formalin-fixed paraffin-embedded pancreatic tissue from autoimmune pancreatitis, chronic pancreatitis, and pancreatic cancer. JOP. 2013 Jul 10; 14(4):405-14. View Abstract
  92. An in-depth comparison of the male pediatric and adult urinary proteomes. Biochim Biophys Acta. 2014 May; 1844(5):1044-50. View Abstract
  93. Cross-species analysis of nicotine-induced proteomic alterations in pancreatic cells. Proteomics. 2013 May; 13(9):1499-1512. View Abstract
  94. Mass spectrometry-based quantitative proteomic profiling of human pancreatic and hepatic stellate cell lines. Genomics Proteomics Bioinformatics. 2013 Apr; 11(2):105-13. View Abstract
  95. Post-translational modifications of pancreatic fluid proteins collected via the endoscopic pancreatic function test (ePFT). J Proteomics. 2013 Oct 30; 92:216-27. View Abstract
  96. Analysis of endoscopic pancreatic function test (ePFT)-collected pancreatic fluid proteins precipitated via ultracentrifugation. JOP. 2013 Mar 10; 14(2):176-86. View Abstract
  97. Carbamylation of serum albumin as a risk factor for mortality in patients with kidney failure. Sci Transl Med. 2013 Mar 06; 5(175):175ra29. View Abstract
  98. Cytosolic 5'-nucleotidase 1A autoimmunity in sporadic inclusion body myositis. Ann Neurol. 2013 Mar; 73(3):408-18. View Abstract
  99. Gas2l3, a novel constriction site-associated protein whose regulation is mediated by the APC/C Cdh1 complex. PLoS One. 2013; 8(2):e57532. View Abstract
  100. SweetSEQer, simple de novo filtering and annotation of glycoconjugate mass spectra. Mol Cell Proteomics. 2013 Jun; 12(6):1735-40. View Abstract
  101. Subcellular fractionation enhances proteome coverage of pancreatic duct cells. Biochim Biophys Acta. 2013 Apr; 1834(4):791-7. View Abstract
  102. A non-parametric cutout index for robust evaluation of identified proteins. Mol Cell Proteomics. 2013 Mar; 12(3):807-12. View Abstract
  103. Short Gel, Long Gradient Liquid Chromatography Tandem Mass Spectrometry to Discover Urinary Biomarkers of Chronic Pancreatitis. Open Proteomics J. 2013; 6:1-13. View Abstract
  104. Urine proteomics for discovery of improved diagnostic markers of Kawasaki disease. EMBO Mol Med. 2013 Feb; 5(2):210-20. View Abstract
  105. Post-translational modification: nature's escape from genetic imprisonment and the basis for dynamic information encoding. Wiley Interdiscip Rev Syst Biol Med. 2012 Nov-Dec; 4(6):565-83. View Abstract
  106. A Src family kinase-Shp2 axis controls RUNX1 activity in megakaryocyte and T-lymphocyte differentiation. Genes Dev. 2012 Jul 15; 26(14):1587-601. View Abstract
  107. Integration analysis of quantitative proteomics and transcriptomics data identifies potential targets of frizzled-8 protein-related antiproliferative factor in vivo. BJU Int. 2012 Dec; 110(11 Pt C):E1138-46. View Abstract
  108. Tiki1 is required for head formation via Wnt cleavage-oxidation and inactivation. Cell. 2012 Jun 22; 149(7):1565-77. View Abstract
  109. Mass Spectrometry-Based (GeLC-MS/MS) Comparative Proteomic Analysis of Endoscopically (ePFT) Collected Pancreatic and Gastroduodenal Fluids. Clin Transl Gastroenterol. 2012 May 03; 3:e14. View Abstract
  110. FLEXIQinase, a mass spectrometry-based assay, to unveil multikinase mechanisms. Nat Methods. 2012 Apr 08; 9(5):504-8. View Abstract
  111. Overcoming species boundaries in peptide identification with Bayesian information criterion-driven error-tolerant peptide search (BICEPS). Mol Cell Proteomics. 2012 Jul; 11(7):M111.014167. View Abstract
  112. Mass spectrometry-based proteomics for translational research: a technical overview. Yale J Biol Med. 2012 Mar; 85(1):59-73. View Abstract
  113. Proteomic analysis of formalin-fixed paraffin-embedded pancreatic tissue using liquid chromatography tandem mass spectrometry. Pancreas. 2012 Mar; 41(2):175-85. View Abstract
  114. Proteomic analysis (GeLC-MS/MS) of ePFT-collected pancreatic fluid in chronic pancreatitis. J Proteome Res. 2012 Mar 02; 11(3):1897-912. View Abstract
  115. Detection and diagnostic value of urine leucine-rich a-2-glycoprotein in children with suspected acute appendicitis. Ann Emerg Med. 2012 Jul; 60(1):78-83.e1. View Abstract
  116. Etiology of limb girdle muscular dystrophy 1D/1E determined by laser capture microdissection proteomics. Ann Neurol. 2012 Jan; 71(1):141-5. View Abstract
  117. A practical guide to the FLEXIQuant method. Methods Mol Biol. 2012; 893:295-319. View Abstract
  118. Optimization of cell lysis and protein digestion protocols for the analysis of HeLa S3 cells by LC-MS/MS. Proteomics. 2011 Dec; 11(24):4726-30. View Abstract
  119. Design and validation of a high-throughput matrix-assisted laser desorption ionization time-of-flight mass spectrometry method for quantification of hepcidin in human plasma. Anal Chem. 2011 Nov 01; 83(21):8357-62. View Abstract
  120. Brainstem deficiency of the 14-3-3 regulator of serotonin synthesis: a proteomics analysis in the sudden infant death syndrome. Mol Cell Proteomics. 2012 Jan; 11(1):M111.009530. View Abstract
  121. Life cycle stage-resolved proteomic analysis of the excretome/secretome from Strongyloides ratti--identification of stage-specific proteases. Mol Cell Proteomics. 2011 Dec; 10(12):M111.010157. View Abstract
  122. mz5: space- and time-efficient storage of mass spectrometry data sets. Mol Cell Proteomics. 2012 Jan; 11(1):O111.011379. View Abstract
  123. Proteomic analysis of a rat pancreatic stellate cell line using liquid chromatography tandem mass spectrometry (LC-MS/MS). J Proteomics. 2011 Dec 21; 75(2):708-17. View Abstract
  124. A polymorphism in the leptin gene promoter is associated with anemia in patients with HIV disease. Blood. 2011 Nov 17; 118(20):5401-8. View Abstract
  125. Proteomic analysis of an immortalized mouse pancreatic stellate cell line identifies differentially-expressed proteins in activated vs nonproliferating cell states. J Proteome Res. 2011 Oct 07; 10(10):4835-44. View Abstract
  126. Stage-specific excretory-secretory small heat shock proteins from the parasitic nematode Strongyloides ratti--putative links to host's intestinal mucosal defense system. FEBS J. 2011 Sep; 278(18):3319-36. View Abstract
  127. Proteome of human perilymph. J Proteome Res. 2011 Sep 02; 10(9):3845-51. View Abstract
  128. Proteomic analysis of palmitoylated platelet proteins. Blood. 2011 Sep 29; 118(13):e62-73. View Abstract
  129. Difference gel electrophoresis identifies differentially expressed proteins in endoscopically collected pancreatic fluid. Electrophoresis. 2011 Aug; 32(15):1939-51. View Abstract
  130. Cytokine profiling of pancreatic fluid using the ePFT collection method in tandem with a multiplexed microarray assay. J Immunol Methods. 2011 Jun 30; 369(1-2):98-107. View Abstract
  131. Comparative analysis of Erk phosphorylation suggests a mixed strategy for measuring phospho-form distributions. Mol Syst Biol. 2011 Apr 12; 7:482. View Abstract
  132. Quantitative proteomics identifies a beta-catenin network as an element of the signaling response to Frizzled-8 protein-related antiproliferative factor. Mol Cell Proteomics. 2011 Jun; 10(6):M110.007492. View Abstract
  133. Mass spectrometry-based proteomics of endoscopically collected pancreatic fluid in chronic pancreatitis research. Proteomics Clin Appl. 2011 Apr; 5(3-4):109-20. View Abstract
  134. libfbi: a C++ implementation for fast box intersection and application to sparse mass spectrometry data. Bioinformatics. 2011 Apr 15; 27(8):1166-7. View Abstract
  135. One-step sample concentration, purification, and albumin depletion method for urinary proteomics. J Proteome Res. 2010 Nov 05; 9(11):6082-9. View Abstract
  136. Iron overload in patients with acute leukemia or MDS undergoing myeloablative stem cell transplantation. Biol Blood Marrow Transplant. 2011 Jun; 17(6):852-60. View Abstract
  137. Proteomic analysis of endoscopically (endoscopic pancreatic function test) collected gastroduodenal fluid using in-gel tryptic digestion followed by LC-MS/MS. Proteomics Clin Appl. 2010 Sep; 4(8-9):715-25. View Abstract
  138. Identification of pancreas-specific proteins in endoscopically (endoscopic pancreatic function test) collected pancreatic fluid with liquid chromatography--tandem mass spectrometry. Pancreas. 2010 Aug; 39(6):889-96. View Abstract
  139. Optimized sample preparation of endoscopic collected pancreatic fluid for SDS-PAGE analysis. Electrophoresis. 2010 Jul; 31(14):2377-87. View Abstract
  140. Quantitative proteomics analysis reveals molecular networks regulated by epidermal growth factor receptor level in head and neck cancer. J Proteome Res. 2010 Jun 04; 9(6):3073-82. View Abstract
  141. Estimating the confidence of peptide identifications without decoy databases. Anal Chem. 2010 Jun 01; 82(11):4314-8. View Abstract
  142. Effect of high-accuracy precursor masses on phosphopeptide identification from MS3 spectra. Anal Chem. 2010 May 15; 82(10):3977-80. View Abstract
  143. MGFp: an open Mascot Generic Format parser library implementation. J Proteome Res. 2010 May 07; 9(5):2762-3. View Abstract
  144. Deuteration distribution estimation with improved sequence coverage for HX/MS experiments. Bioinformatics. 2010 Jun 15; 26(12):1535-41. View Abstract
  145. Phosphoproteomics. Wiley Interdiscip Rev Syst Biol Med. 2010 May-Jun; 2(3):255-276. View Abstract
  146. Extensive phosphorylation with overlapping specificity by Mycobacterium tuberculosis serine/threonine protein kinases. Proc Natl Acad Sci U S A. 2010 Apr 20; 107(16):7521-6. View Abstract
  147. High-throughput matrix-assisted laser desorption ionization-time-of-flight mass spectrometry method for quantification of hepcidin in human urine. Anal Chem. 2010 Feb 15; 82(4):1551-5. View Abstract
  148. Non-linear classification for on-the-fly fractional mass filtering and targeted precursor fragmentation in mass spectrometry experiments. Bioinformatics. 2010 Mar 15; 26(6):791-7. View Abstract
  149. When less can yield more - Computational preprocessing of MS/MS spectra for peptide identification. Proteomics. 2009 Nov; 9(21):4978-84. View Abstract
  150. Computational protein profile similarity screening for quantitative mass spectrometry experiments. Bioinformatics. 2010 Jan 01; 26(1):77-83. View Abstract
  151. Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes. Mol Cell Proteomics. 2010 Jan; 9(1):54-70. View Abstract
  152. Binding partner switching on microtubules and aurora-B in the mitosis to cytokinesis transition. Mol Cell Proteomics. 2010 Feb; 9(2):336-50. View Abstract
  153. Urine proteomics for profiling of human disease using high accuracy mass spectrometry. Proteomics Clin Appl. 2009 Sep 01; 3(9):1052-1061. View Abstract
  154. Molecular and functional characterisation of the heat shock protein 10 of Strongyloides ratti. Mol Biochem Parasitol. 2009 Dec; 168(2):149-57. View Abstract
  155. Proteomics and opportunities for clinical translation in urological disease. J Urol. 2009 Sep; 182(3):835-43. View Abstract
  156. Discovery and validation of urine markers of acute pediatric appendicitis using high-accuracy mass spectrometry. Ann Emerg Med. 2010 Jan; 55(1):62-70.e4. View Abstract
  157. Differentiation-dependent interactions between RUNX-1 and FLI-1 during megakaryocyte development. Mol Cell Biol. 2009 Aug; 29(15):4103-15. View Abstract
  158. FLEXIQuant: a novel tool for the absolute quantification of proteins, and the simultaneous identification and quantification of potentially modified peptides. J Proteome Res. 2009 May; 8(5):2201-10. View Abstract
  159. The mechanism and pattern of yolk consumption provide insight into embryonic nutrition in Xenopus. Development. 2009 May; 136(9):1539-48. View Abstract
  160. A simple and effective method for detecting phosphopeptides for phosphoproteomic analysis. J Proteomics. 2009 Jul 21; 72(5):831-5. View Abstract
  161. Urine proteomics for profiling of human disease using high accuracy mass spectrometry. Proteomics Clin Appl. 2009; 3:1052-61. View Abstract
  162. NITPICK: peak identification for mass spectrometry data. BMC Bioinformatics. 2008 Aug 28; 9:355. View Abstract
  163. Robust prediction of the MASCOT score for an improved quality assessment in mass spectrometric proteomics. J Proteome Res. 2008 Sep; 7(9):3708-17. View Abstract
  164. Optimizing sample handling for urinary proteomics. J Proteome Res. 2008 Sep; 7(9):4022-30. View Abstract
  165. Rapid preparation of nuclei-depleted detergent-resistant membrane fractions suitable for proteomics analysis. BMC Cell Biol. 2008 Jun 05; 9:30. View Abstract
  166. Alkaline Phosphatase Treatment of Phosphopeptides: In-Solution Dephosphorylation prior to MALDI-MS Analysis. CSH Protoc. 2008 May 01; 2008:pdb.prot4610. View Abstract
  167. Alkaline Phosphatase Treatment of Phosphopeptides: In-Solution Dephosphorylation after MALDI-MS Analysis. CSH Protoc. 2008 May 01; 2008:pdb.prot4611. View Abstract
  168. Alkaline Phosphatase Treatment of Phosphopeptides: On-Probe Dephosphorylation after MALDI-MS Analysis. CSH Protoc. 2008 May 01; 2008:pdb.prot4612. View Abstract
  169. Different phosphorylation states of the anaphase promoting complex in response to antimitotic drugs: a quantitative proteomic analysis. Proc Natl Acad Sci U S A. 2008 Apr 22; 105(16):6069-74. View Abstract
  170. SALAMI (Spectrum ALignments using high Accuracy Mass and hIgh sensitivity data): how to make the best out of hybrid MS/MS data. J Proteome Res. 2008 May; 7(5):1984-93. View Abstract
  171. Temporal variations of the postnatal rat urinary proteome as a reflection of systemic maturation. Proteomics. 2008 Mar; 8(5):1097-112. View Abstract
  172. Proteomic approaches to the analysis of multiprotein signaling complexes. Proteomics. 2008 Feb; 8(4):832-51. View Abstract
  173. Phosphopeptide Purification by IMAC with Fe(III) and Ga(III). CSH Protoc. 2007 Nov 01; 2007:pdb.prot4607. View Abstract
  174. Preparation and Use of Microcolumns for Sample Desalting or Nanoscale IMAC. CSH Protoc. 2007 Nov 01; 2007:pdb.prot4608. View Abstract
  175. amsrpm: Robust Point Matching for Retention Time Alignment of LC/MS Data with R. J Stat Software. 2007; 18:4. View Abstract
  176. Why b, y’s? Sodiation-induced tryptic peptide-like fragmentation of non-tryptic peptides. Int J Mass Spectrom. 2007; 268:181-189. View Abstract
  177. Protein sulfation analysis--A primer. Biochim Biophys Acta. 2006 Dec; 1764(12):1904-13. View Abstract
  178. A quantitative proteomic analysis of growth factor-induced compositional changes in lipid rafts of human smooth muscle cells. Proteomics. 2005 Dec; 5(18):4733-42. View Abstract
  179. Phosphorylation analysis by mass spectrometry: myths, facts, and the consequences for qualitative and quantitative measurements. Mol Cell Proteomics. 2006 Jan; 5(1):172-81. View Abstract
  180. Stable isotope-free relative and absolute quantitation of protein phosphorylation stoichiometry by MS. Proc Natl Acad Sci U S A. 2005 Mar 15; 102(11):3948-53. View Abstract
  181. Purification of active TFIID from Saccharomyces cerevisiae. Extensive promoter contacts and co-activator function. J Biol Chem. 2004 Nov 26; 279(48):49973-81. View Abstract
  182. The ABC's (and XYZ's) of peptide sequencing. Nat Rev Mol Cell Biol. 2004 Sep; 5(9):699-711. View Abstract
  183. Autophosphorylation of JAK2 on tyrosines 221 and 570 regulates its activity. Mol Cell Biol. 2004 Jun; 24(11):4955-67. View Abstract
  184. Purification and architecture of the ubiquitous Wave complex. Proc Natl Acad Sci U S A. 2004 Mar 30; 101(13):4379-83. View Abstract
  185. Development of human protein reference database as an initial platform for approaching systems biology in humans. Genome Res. 2003 Oct; 13(10):2363-71. View Abstract
  186. Protein profiling with cleavable isotope-coded affinity tag (cICAT) reagents: the yeast salinity stress response. Mol Cell Proteomics. 2003 Nov; 2(11):1198-204. View Abstract
  187. Using optimized collision energies and high resolution, high accuracy fragment ion selection to improve glycopeptide detection by precursor ion scanning. J Am Soc Mass Spectrom. 2003 Jul; 14(7):777-84. View Abstract
  188. Phosphotyrosine mapping in Bcr/Abl oncoprotein using phosphotyrosine-specific immonium ion scanning. Mol Cell Proteomics. 2003 Mar; 2(3):138-45. View Abstract
  189. Analysis of bromotryptophan and hydroxyproline modifications by high-resolution, high-accuracy precursor ion scanning utilizing fragment ions with mass-deficient mass tags. Anal Chem. 2002 Dec 15; 74(24):6230-6. View Abstract
  190. Analysis of tyrosine phosphorylation sites in signaling molecules by a phosphotyrosine-specific immonium ion scanning method. Sci STKE. 2002 Oct 15; 2002(154):pl16. View Abstract
  191. Proteomics goes quantitative: measuring protein abundance. Trends Biotechnol. 2002 Sep; 20(9):361-4. View Abstract
  192. A new derivatization strategy for the analysis of phosphopeptides by precursor ion scanning in positive ion mode. J Am Soc Mass Spectrom. 2002 Aug; 13(8):996-1003. View Abstract
  193. Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome. Trends Biotechnol. 2002 Jun; 20(6):261-8. View Abstract
  194. Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. Mol Cell Proteomics. 2002 May; 1(5):376-86. View Abstract
  195. Analysis of protein-nucleic acid interactions by photochemical cross-linking and mass spectrometry. Mass Spectrom Rev. 2002 May-Jun; 21(3):163-82. View Abstract
  196. Multi-Protein Complexes Studied by Mass Spectrometry. ScientificWorldJournal. 2002; 2:91-92. View Abstract
  197. Directed proteomic analysis of the human nucleolus. Curr Biol. 2002 Jan 08; 12(1):1-11. View Abstract
  198. Loss of chromosome 11q21-23.1 and 17p and gain of chromosome 6p are independent prognostic indicators in B-cell non-Hodgkin's lymphoma. Br J Cancer. 2001 Dec 14; 85(12):1900-13. View Abstract
  199. GPMAW--a software tool for analyzing proteins and peptides. Trends Biochem Sci. 2001 Nov; 26(11):687-9. View Abstract
  200. Tyrosine phosphorylation mapping of the epidermal growth factor receptor signaling pathway. J Biol Chem. 2002 Jan 11; 277(2):1031-9. View Abstract
  201. Mass spectrometric analysis of a UV-cross-linked protein-DNA complex: tryptophans 54 and 88 of E. coli SSB cross-link to DNA. Protein Sci. 2001 Oct; 10(10):1989-2001. View Abstract
  202. Quadrupole time-of-flight versus triple-quadrupole mass spectrometry for the determination of phosphopeptides by precursor ion scanning. J Mass Spectrom. 2001 Jul; 36(7):782-90. View Abstract
  203. Labile sulfogroup allows differentiation of sulfotyrosine and phosphotyrosine in peptides. J Mass Spectrom. 2001 Jul; 36(7):832-3. View Abstract
  204. Investigation of tyrosine nitration in proteins by mass spectrometry. J Mass Spectrom. 2001 Jun; 36(6):616-25. View Abstract
  205. Detection of tyrosine phosphorylated peptides by precursor ion scanning quadrupole TOF mass spectrometry in positive ion mode. Anal Chem. 2001 Apr 01; 73(7):1440-8. View Abstract
  206. Molecular patterning of the oikoplastic epithelium of the larvacean tunicate Oikopleura dioica. J Biol Chem. 2001 Jun 08; 276(23):20624-32. View Abstract
  207. Similarity between condensed phase and gas phase chemistry: fragmentation of peptides containing oxidized cysteine residues and its implications for proteomics. J Am Soc Mass Spectrom. 2001 Feb; 12(2):228-32. View Abstract
  208. Mass Spectrometry for the Analysis of Protein Modifications (Ph.D. Thesis). 2001. View Abstract
  209. Identification of a novel immunoreceptor tyrosine-based activation motif-containing molecule, STAM2, by mass spectrometry and its involvement in growth factor and cytokine receptor signaling pathways. J Biol Chem. 2000 Dec 08; 275(49):38633-9. View Abstract
  210. Gap junctional intercellular communication is not a major mediator in the bystander effect in photodynamic treatment of MDCK II cells. Radiat Res. 2000 Sep; 154(3):331-41. View Abstract
  211. The bystander effect in photodynamic inactivation of cells. Biochim Biophys Acta. 2000 Jul 26; 1475(3):273-80. View Abstract
  212. Oncogenic aberrations in the p53 pathway are associated with a high S phase fraction and poor patient survival in B-cell Non-Hodgkin's lymphoma. Int J Cancer. 2000 Jul 20; 89(4):313-24. View Abstract
  213. Detection and quantification of neurotensin in human brain tissue by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Chem. 2000 Jul 15; 72(14):3320-6. View Abstract
  214. Phenyl thiocarbamoyl and related derivatives of peptides: Edman chemistry in the gas phase. Int J Mass Spectrom. 1999; 188:95-103. View Abstract
  215. The SHi Reaction at Silicon – A New Entry into Cyclic Alkoxy-silanes. Chem Eur J. 1999; 5:759-73. View Abstract
  216. Stereoselective Phenyl Migration from Silicon to Carbon. Tetrahedron Lett. 1998; 39:8829-32. View Abstract
  217. Intramolecular Homolytic Substitution on Silicon – Influence of the Degree of Substitution on the Rate of Cyclisation (Dipl. Chem. Thesis). 1998. View Abstract
  218. Techniques for the Mass Spectrometric Localisation of Double Bonds (B.Sc. Thesis). 1995. View Abstract
  219. The initiation mass for DNA replication in Escherichia coli K-12 is dependent on growth rate. EMBO J. 1994 May 01; 13(9):2097-102. View Abstract
  220. Characterization of singlet oxygen-induced guanine residue damage after photochemical treatment of free nucleosides and DNA. Biochim Biophys Acta. 1994 Jan 18; 1217(1):9-15. View Abstract
  221. Plateau distributions of DNA fragment lengths produced by extended light exposure of extranuclear photosensitizers in human cells. Nucleic Acids Res. 1992 Dec 25; 20(24):6687-93. View Abstract
  222. Differential chromatin structure-dependent binding of 7-aminoactinomycin D in normal and malignant bone marrow hematopoietic cells. Cancer Res. 1992 Sep 15; 52(18):5007-12. View Abstract
  223. Synergistic effects of photoactivated tetra(4-sulfonatophenyl)porphine and nocodazole on microtubule assembly, accumulation of cells in mitosis and cell survival. J Photochem Photobiol B. 1992 Apr 15; 13(1):59-70. View Abstract
  224. Cell cycle-specific expression and nuclear binding of DNA polymerase alpha. Mol Cell Biol. 1991 Jun; 11(6):3384-9. View Abstract
  225. Fluorescence spectra of Hoechst 33258 bound to chromatin. Biochim Biophys Acta. 1986 Oct 16; 868(1):17-23. View Abstract
  226. Timing of initiation of chromosome replication in individual Escherichia coli cells. EMBO J. 1986 Jul; 5(7):1711-7. View Abstract
  227. Neither adriamycin nor actinomycin D displaces Tb3+ from DNA. Biochim Biophys Acta. 1985 Aug 21; 825(4):416-8. View Abstract
  228. Promotive and inhibitory effects of macrophages on the mitogen-induced blastogenesis of lymphocytes: a flow-cytometric study. Scand J Immunol. 1981 Jul; 14(1):39-46. View Abstract
  229. Further developments of a microscope-based flow cytometer: light scatter detection and excitation intensity compensation. Cytometry. 1980 Jul; 1(1):26-31. View Abstract
  230. Molecular excitations in radiation damage as studies by the radiation-induced luminescence of proteins. Scand J Clin Lab Invest Suppl. 1968; 106:21-30. View Abstract

Contact Hanno Steen